Laboratory of Pharmacology and Brain Biostructure

Research profile
The research interests of the Laboratory of Pharmacology and Brain Biostructure are centered on neurodevelopmental models of schizophrenia. Specifically, our investigations are aimed at identifying the neural substrates underlying behavioral deficits typical for schizophrenia and the discovery of novel potential targets for antipsychotic substances. Another important aspect of our research involves environmental factors (early life stress, enriched environment) that influence brain development and its susceptibility to mental disorders such as anxiety, schizophrenia, cognitive deficits and depression.

Research methods
Behavioral: neurodevelopmental models of schizophrenia, experimental models of stress – maternal separation in the postnatal period, fear conditioning paradigm –  the acquisition, retention and extinction of memory traces, sensory motor gating paradigm, novel object recognition test, delayed alternation test, social interaction test, food-preference test, latent inhibition test.
Immunohistochemistry and neuroanatomy: brain tissue staining for identification of specific proteins and their colocalization - microscopic techniques based on light microscopy, fluorescence and laser confocal microscopy, stereological techniques. Stereotactic and iontophoretic techniques of retrograde marker delivery. Methods to assess adult brain neurogenesis.
Biochemistry and molecular biology: protein expression: Western blot, ELISA, protein macroarrays; Real-Time Quantitative Reverse Transcription,  chromatin precipitation.
Electrophysiology: in vitro measurements of long-term synaptic potentiation and depression.

More publications
  • The role of endoplasmic reticulum stress in the pathomechanisms of early-life stressinduced dysfunction of the prefronatal cortex and in the mechanisms of action of fluoxetine in children and adolescents, Agnieszka Chocyk, PhD

  • Impact of early-life stress on maturation, permeability of blood brain-barrier and neuroinflammatory processes activation during ontogenetic development , Anna Solarz, MSc

    Preludium 12 2016/23/N/NZ4/01148 - kierownik

  • Changes in neurogenesis during withdrawal from nicotine self-administration in rats - role of serotonin (5-HT)2C receptors, Magdalena Zaniewska, PhD

    NCN GRANT nr UMO-2015/17/B/NZ4/02621 (OPUS 9) 

  • , Agnieszka Chocyk, PhD
  • Defining the mechanism regulating serotonin function in the development of depression-like behaviour and alcohol addiction in animals., Magdalena Zaniewska, PhD

    Project ID: 632/MOB/2011/0

    Place of Project implementation: MDC, Molecular Biology of Peptide Hormones, Berlin, Germany

  • Signalling cascades underlying behavioural effects of nicotine in rats, Magdalena Zaniewska, PhD

    Project ID: A/10/05975 Referat 323

    Places of project implementation: Max Delbrück Center for Molecular Medicine (MDC), Molecular Biology of Peptide Hormones, Berlin, Germany

  • , Agnieszka Chocyk, PhD
  • , Agnieszka Chocyk, PhD
  • , Agnieszka Chocyk, PhD
  • , Agnieszka Chocyk, PhD