Department of Drug Addiction Pharmacology
Head:
Professor Małgorzata Filip
Research staff:
Professor Edmund Przegaliński MD PhD, Małgorzata Frankowska PhD, Karolina Wydra PhD, Magdalena Hubalewska-Mazgaj PhD, Irena Smaga-Maślanka PhD
Technical staff / specialists:
Teresa Piwowarczyk, Agata Suder, Renata Pieniążek
PhD students:
Justyna Brodowicz MSc, Dawid Gawliński MSc, Kinga Gawlińska MSc
Research profile
The Department of Drug Addiction Pharmacology (DAP) was founded in 2017 as a continuation of Drug Addiction Laboratory (established in 2006 as a unit in the Department of Pharmacology). The team is focused on studying various aspects of addiction to psychostimulants (amphetamine, methylenedioxyamphetamine, cocaine), nicotine, alcohol and THC. Study areas of the DAP include molecular and neurochemical mechanisms of addiction and possibilities for pharmacological intervention.
In our research, we use virtually all available research methods that visualize different stages of drug addiction process (initiation, prolonged drug use, craving and other withdrawal symptoms, relapses). The scope of our research includes animal models (experimental depression, transgenic animals), pharmacological manipulations (e.g. lesions, intrastructural and peripheral drug application, injections of selective receptor ligands), biochemical analyzes (changes in receptor and enzyme expression, synaptic and tissue levels of neurotransmitters / neuromodulators) and molecular analysis (mitochondrial DNA (mtDNA), gene and miRNA expression, epigenetic analysis).
We closely cooperate with a number of scientific units in Poland (Department of Pharmacodynamics, Faculty of Pharmacy - Medical University of Warsaw, Department of Pharmacology and Pharmacodynamics, Faculty of Pharmacy - Medical University of Lublin) and abroad (Department of Nervous System Research - Karolinska Institutet, Faculty of Pharmacy - University of Ferrara, Department of Pharmacological and Biomolecular Research - University of Milan, Department of Psychiatry and Psychotherapy - University of Friedrich Alexander in Erlangen-Nürnberg).
Research methods
Behavioral methods and tests:
Measurement of locomotor activity in animals, intravenous self-administration of addictive substances, free choice alcohol consumption, natural reward self-administration, sucrose preference test, drug discrimination test, elevated zero maze, modified forced swim test
Molecular biology methods:
DNA / RNA isolation, PCR, RT-qPCRe, electrophoresis in agarose and polyacrylamide gels
Biochemical methods:
Protein isolation, BCA protein assay, Western blot, ELISA, in vivo microdialysis
Histological and histochemical methods:
Tissue fixation, receptor autoradiography, immunohistochemistry and immunofluorescence
Other:
Stereotactic implantation of intracerebral cannulas, intravenous cannulation, intracerebral drug administration, bulbectomy
The most important discoveries in the last 3 years
- Amide derivative of N-acetylcysteine inhibits cocaine relapse in rats with experimental depression.
- Tonic activation of A2A receptors located in the nucleus accumbens induces cocaine relapse.
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The Positive and Negative Outcome of Morphine and Disulfiram Subacute Co-Administration in Rats in the Absence of Ethanol Challenge.
Karolina Frączek, Agnieszka Kowalczyk, Martyna Pekala, Kaja Kasarello, Grażyna Sygitowicz, Dorota Sulejczak, Malgorzata Zaremba, Marek Konop, Malgorzata Frankowska, Malgorzata Filip, Magdalena Bujalska-Zadrozny, Patrycja Kleczkowska
Pharmaceutics, E29 10.3390/pharmaceutics13010029
PMID:33375279 -
Cocaine use disorder: A look at metabotropic glutamate receptors and glutamate transporters.
Ewa Niedzielska-Andres, Lucyna Pomierny-Chamioło, Michał Andres, Maria Walczak, Lori A Knackstedt, Małgorzata Filip, Edmund Przegaliński
Pharmacology & therapeutics, S0163-7258(20)30328-4 10.1016/j.pharmthera.2020.107797
PMID:33359590 -
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M Frankowska, J Miszkiel, L Pomierny-Chamiolo, B Pomierny, A Celeste Borelli, A Suder, M Filip
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 10.26402/jpp.2020.4.09
PMID:33316768 -
Maternal dietary patterns are associated with susceptibility to a depressive-like phenotype in rat offspring.
Kinga Gawlińska, Dawid Gawliński, Michał Korostyński, Małgorzata Borczyk, Małgorzata Frankowska, Marcin Piechota, Małgorzata Filip, Edmund Przegaliński
Developmental cognitive neuroscience, S1878-9293(20)30129-8 10.1016/j.dcn.2020.100879
PMID:33232913 -
Cocaine Administration and Its Abstinence Conditions Modulate Neuroglia.
Kinga Gawlińska, Małgorzata Frankowska, Dawid Gawliński, Marcin Piechota, Michał Korostyński, Małgorzata Filip
International journal of molecular sciences, E7970 10.3390/ijms21217970
PMID:33120991 -
Extinction Training after Cocaine Self-Administration Influences the Epigenetic and Genetic Machinery Responsible for Glutamatergic Transporter Gene Expression in Male Rat Brain.
Irena Smaga, Kinga Gawlińska, Małgorzata Frankowska, Karolina Wydra, Anna Sadakierska-Chudy, Agata Suder, Marcin Piechota, Małgorzata Filip
Neuroscience, S0306-4522(20)30654-0 10.1016/j.neuroscience.2020.10.001
PMID:33065231 -
Neutral Sphingomyelinase is an Affective Valence-Dependent Regulator of Learning and Memory.
Liubov S Kalinichenko, Laila Abdel-Hafiz, An-Li Wang, Christiane Mühle, Nadine Rösel, Fabian Schumacher, Burkhard Kleuser, Irena Smaga, Malgorzata Frankowska, Malgorzata Filip, Gerd Schaller, Tanja Richter-Schmidinger, Bernd Lenz, Erich Gulbins, Johannes Kornhuber, André W C Oliveira, Marilia Barros, Joseph P Huston, Christian P Müller
Cerebral cortex (New York, N.Y. : 1991), bhaa298 10.1093/cercor/bhaa298
PMID:33043975 -
Cocaine attenuates acid sphingomyelinase activity during establishment of addiction-related behavior-A translational study in rats and monkeys.
Małgorzata Frankowska, Fernando M Jesus, Christiane Mühle, Jéssica V N Pacheco, Rafael S Maior, Anna Sadakierska-Chudy, Irena Smaga, Marcin Piechota, Liubov S Kalinichenko, Erich Gulbins, Johannes Kornhuber, Małgorzata Filip, Christian P Müller, Marilia Barros
Addiction biology, 10.1111/adb.12955
PMID:32761719 -
Cocaine Self-Administration and Abstinence Modulate NMDA Receptor Subunits and Active Zone Proteins in the Rat Nucleus Accumbens.
Irena Smaga, Karolina Wydra, Małgorzata Frankowska, Fabio Fumagalli, Marek Sanak, Małgorzata Filip
Molecules (Basel, Switzerland), E3480 10.3390/molecules25153480
PMID:32751823 -
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Małgorzata Frankowska, Joanna Miszkiel, Lucyna Pomierny-Chamioło, Bartosz Pomierny, Andrea Celeste Borelli, Agata Suder, Małgorzata Filip
Pharmacological reports : PR, 10.1007/s43440-020-00129-8
PMID:32691344 -
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Kinga Gawlińska, Joanna Jastrzębska, Sara Gamberini, Dawid Gawliński, Renata Pieniążek, Agata Suder, Karolina Wydra, Małgorzata Frankowska
European journal of pharmacology, S0014-2999(20)30416-7 10.1016/j.ejphar.2020.173324
PMID:32621910 -
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Karolina Wydra, Dawid Gawliński, Kinga Gawlińska, Małgorzata Frankowska, Dasiel O Borroto-Escuela, Kjell Fuxe, Małgorzata Filip
Cells, E1372 10.3390/cells9061372
PMID:32492952 -
Molecular changes evoked by the beta-lactam antibiotic ceftriaxone across rodent models of substance use disorder and neurological disease.
Irena Smaga, Daniel Fierro, Javier Mesa, Malgorzata Filip, Lori A Knackstedt
Neuroscience and biobehavioral reviews, S0149-7634(20)30419-X 10.1016/j.neubiorev.2020.05.016
PMID:32485268 -
Relationship of maternal high-fat diet during pregnancy and lactation to offspring health.
Kinga Gawlińska, Dawid Gawliński, Małgorzata Filip, Edmund Przegaliński
Nutrition reviews, nuaa020 10.1093/nutrit/nuaa020
PMID:32447401 -
Maternal Diet Influences the Reinstatement of Cocaine-Seeking Behavior and the Expression of Melanocortin-4 Receptors in Female Offspring of Rats.
Dawid Gawliński, Kinga Gawlińska, Małgorzata Frankowska, Małgorzata Filip
Nutrients, E1462 10.3390/nu12051462
PMID:32438560
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, Professor Małgorzata Filip, PhD
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, Professor Małgorzata Filip, PhD
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Effects and potential mechanisms of action of dislufiram and nepicastat in morphine dependence in laboratory animal , Małgorzata Frankowska, PhD
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, Professor Małgorzata Filip, PhD
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The role of the mGlu5-D2 heterodimeric receptor complexes in the extinction of drug seeking behavior associated with dependents to psichostimulants (cocaine and MDMA): behavioral and biomolecular analyses in rats , Małgorzata Frankowska, PhD
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Understanding cocaine addiction. A2A-D2 and A1-D1 heteromers as novel targets for cocaine action, Małgorzata Frankowska, PhD
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, Irena Smaga-Maślanka, PhD
Tuesday, 9 June 2020 -
, Małgorzata Frankowska, PhD
Thursday, 20 December 2018 -
Awarded internship founded by Leading National Research Center (KNOW), Institute of Pharmacology PAS, Małgorzata Frankowska, PhD
Wednesday, 8 February 2017 -
, Irena Smaga-Maślanka, PhD
Monday, 30 November -0001 -
, Irena Smaga-Maślanka, PhD
Monday, 30 November -0001
techniques
- cDNA microarrays
- drug self administration
- electrophoresis
- ELISA
- genotyping
- immunofluorescence
- immunohistochemistry
- jugular vein implantation
- liquid chromatography
- locomotor activity
- microdialysis in vitro
- next-generation sequencing
- novel obiect recognition
- protein fractioning
- quantitative autoradiography
- Real Time PCR
- stereotactic implantation of cannulas
- Western Blot
keywords
- adenosine
- allosteric modulation
- animal models
- autism
- behavioural analysis
- CB1 receptor
- cocaine
- cognitive functions
- depression
- dopamine
- drug addiction
- enriched environment
- epigenetics
- frontal cortex
- G-protein coupled receptors
- GABAB receptors
- MDMA
- molecular biology
- motivation
- neuropeptides
- pharmacotherapy
- sex