Department of Neurobiology
The Department of Neurobiology consists of separate research teams and the independent Laboratory of Trace Elements Neurobiology. Research teams conduct complementary studies in areas such as metabotropic receptors for glutamate and the search for new sites of action for psychotropic drugs.
The team consisting of the following members: dr hab. Agnieszka Pałucha-Poniewiera (PhD), Karolina Podkowa (MPharm) and Szymon Rzeźniczek (MSc) takes part in the study of potential antidepressant properties of glutamate metabotropic ligands and their mechanisms of action.
The team consisting of the following members: Piotr Brański (PhD), Grzegorz Burnat (PhD), Paulina Chorobik (PhD), Marcin Marcinak (PhD), Barbara Chruścicka (MSc) deals with an identification and pharmacodynamic characteristics of biologically active chemical compounds. Their studies are focused on metabotropic receptors, in particular mGluR and GABA B.
The methodology and experimental procedures:
- Mammalian cell cultures
- Analysis of protein expression using RT-PCR and Western Blot
- Immunofluorescence (microscopy and FACS)
- Measurement of metabotropic receptors-mediated GTPase activity in isolated cell membranes
- Cyclic ATP measurement (cells, tissue sections) using HTRF
- Kinetic luminometric measurement of intracellular cAMP levels using a molecular sensor (Glo)
- Measurement of the IP1 accumulation (cells, tissue sections) using HTRF
- Measurement of intracellular mobilization of calcium ions (fluorescence)
- Molecular cloning (propagation of bacterial vectors, modification of DNA sequence, transfer into mammalian cells [including tetracycline-inducible gene expression system], selection of cell clones)
- Point mutations
- Experiments on transgenic animals (PCR, electrophoretic separation of products)
- Evaluation of sperm quality parameters (evaluation of: motility, vitality – eosinophilic test, maturity – presence and location of cytoplasmic droplets, integrity of cytoplasmic membrane – hyposmotic test and morphology of spermatozoids)
Dr hab. Joanna Wierońska (PhD), Monika Woźniak (MSc) and Paulina Cieślik (MSc) form a team whose main research direction are antipsychotics and their mechanisms of action. We use animal models of positive, negative and cognitive symptoms as well as techniques to determine the level of specific proteins and mRNA. Our studies oscillate around metabotropic receptors for glutamate and GABA B receptors.
The Laboratory of Neurodegeneration and Neuroprotection (no formal status), whose members are prof. dr hab. Maria Śmiałowska and Dr. Helena Domin, is involved in:
- Research on neurodegeneration and neuroprotection in experimental models of ischemia
- Study of neuroprotective and antidepressant mechanisms of glutamate group III receptor ligands and neuropeptide Y receptor ligands
- Study of anatomical and histochemical interactions between different types of neurons and astrocytes in the cerebral cortex and limbic structures. We pay special attention to GABA and NPY neurons.
- Histology (Nissl, HE, Kluver-Barrera staining)
- Histochemistry and immunohistochemistry (NPY-ir, GAD67-ir, GFAP-ir, CRF-ir, NeuN-ir, TH-ir, TrpH-ir, immunohistochemical staining of selected receptors, and others)
- Stereological quantification in microscopic images
- Intracerebral administration of substances
- Behavioral observations and tests
- Western blot analysis of selected proteins or peptides.
The team closely collaborates with Laboratories of the Department of Neurobiology and other researchers in the Institute, especially in the field of behavior, Western blotting, neural cell cultures, and assessment of neurodegenerative changes in Parkinson's disease models.
The Department also conducts studies regarding the anxiolytic activity of drugs and the mechanisms of action of these compounds.
Grzegorz Ślifirski, Marek Król, Jerzy Kleps, Piotr Podsadni, Mariusz Belka, Tomasz Bączek, Agata Siwek, Katarzyna Stachowicz, Bernadeta Szewczyk, Gabriel Nowak, Andrzej Bojarski, Anna E Kozioł, Jadwiga Turło, Franciszek Herold
European journal of medicinal chemistry, S0223-5234(19)30649-X 10.1016/j.ejmech.2019.07.027
Characterization of the Brain Penetrant Neuropeptide Y Y2 Receptor Antagonist SF-11.
Helena Domin, Natalia Piergies, Ewa Pięta, Elżbieta Wyska, Bartłomiej Pochwat, Piotr Wlaź, Maria Śmiałowska, Czesława Paluszkiewicz, Bernadeta Szewczyk
ACS chemical neuroscience, 10.1021/acschemneuro.9b00082
Behavioral consequences of co-administration of MTEP and the COX-2 inhibitor NS398 in mice. Part 1.
Behavioural brain research, S0166-4328(18)31729-7 10.1016/j.bbr.2019.111961
The role of DSCAM in the regulation of synaptic plasticity: possible involvement in neuropsychiatric disorders.
Acta neurobiologiae experimentalis,
Synthesis of novel pyrido[1,2-c]pyrimidine derivatives with rigidized tryptamine moiety as potential SSRI and 5-HT1A receptor ligands.
Grzegorz Ślifirski, Marek Król, Jerzy Kleps, Szymon Ulenberg, Mariusz Belka, Tomasz Bączek, Agata Siwek, Katarzyna Stachowicz, Bernadeta Szewczyk, Gabriel Nowak, Andrzej Bojarski, Anna E Kozioł, Jadwiga Turło, Franciszek Herold
European journal of medicinal chemistry, S0223-5234(19)30041-8 10.1016/j.ejmech.2019.01.031
Neuroprotective effect of the group III mGlu receptor agonist ACPT-I after ischemic stroke in rats with essential hypertension.
Helena Domin, Łukasz Przykaza, Ewa Kozniewska, Paweł M Boguszewski, Maria Śmiałowska
Progress in neuro-psychopharmacology & biological psychiatry, S0278-5846(17)30736-4 10.1016/j.pnpbp.2018.02.006
Comparative effectiveness of abatacept, apremilast, secukinumab and ustekinumab treatment of psoriatic arthritis: a systematic review and network meta-analysis.
P Kawalec, P Holko, P Moćko, A Pilc
Rheumatology international, 10.1007/s00296-017-3919-7
Involvement of extracellular signal-regulated kinase (ERK) in the short and long-lasting antidepressant-like activity of NMDA receptor antagonists (zinc and Ro 25-6981) in the forced swim test in rats.
Bartłomiej Pochwat, Anna Rafało-Ulińska, Helena Domin, Paulina Misztak, Gabriel Nowak, Bernadeta Szewczyk
Neuropharmacology, S0028-3908(17)30373-8 10.1016/j.neuropharm.2017.08.006
The involvement of monoaminergic neurotransmission in the antidepressant-like action of scopolamine in the tail suspension test.
Agnieszka Pałucha-Poniewiera, Karolina Podkowa, Tomasz Lenda, Andrzej Pilc
Progress in neuro-psychopharmacology & biological psychiatry, S0278-5846(17)30220-8 10.1016/j.pnpbp.2017.06.022
Repeated co-treatment with antidepressants and risperidone increases BDNF mRNA and protein levels in rats.
Zofia Rogóż, Katarzyna Kamińska, Patrycja Pańczyszyn-Trzewik, Magdalena Sowa-Kućma
Pharmacological reports : PR, S1734-1140(16)30398-X 10.1016/j.pharep.2017.02.022
Vortioxetine: A review of the pharmacology and clinical profile of the novel antidepressant.
Magdalena Sowa-Kućma, Patrycja Pańczyszyn-Trzewik, Paulina Misztak, Rafał R Jaeschke, Katherine Sendek, Krzysztof Styczeń, Wojciech Datka, Magdalena Koperny
Pharmacological reports : PR, S1734-1140(16)30361-9 10.1016/j.pharep.2017.01.030
Disease activity, quality of life, and indirect costs of ulcerative colitis in Poland.
Paweł Kawalec, Ewa Stawowczyk, Małgorzata Mossakowska, Andrzej Pilc
Przeglad gastroenterologiczny, 10.5114/pg.2017.66324
Safety Profile of Biologic Drugs in the Treatment of Inflammatory Bowel Diseases: A Systematic Review and Network Meta-analysis of Randomized Controlled Trials.
Paweł Moćko, Paweł Kawalec, Andrzej Pilc
Clinical drug investigation, 10.1007/s40261-016-0459-y
Serotonin transporter and receptor ligands with antidepressant activity as neuroprotective and proapoptotic agents.
Zdzislaw Chilmonczyk, Andrzej J Bojarski, Andrzej Pilc, Ingebrigt Sylte
Pharmacological reports : PR, S1734-1140(16)30162-1 10.1016/j.pharep.2017.01.011
Involvement of GABAB Receptor Signaling in Antipsychotic-like Action of the Novel Orthosteric Agonist of the mGlu4 Receptor, LSP4-2022.
Monika Woźniak, Francine Acher, Marcin Marciniak, Magdalena Lasoń-Tyburkiewicz, Piotr Gruca, Mariusz Papp, Andrzej Pilc, Joanna M Wierońska
Adhesion in the world of plants and animals
"Wszechświat" vol. 120, No. 4-6 / 2019
The article describes the physical phenomenon of adhesion, discussing factors affecting adhesion such as surface tension. Next, the article shows where in the world of plants or animals we can observe adhesion. Finally, the practical use of the above knowledge is discussed.
Down Syndrome Cell Adhesion Molecule (DSCAM)
"Wszechświat" 118, 10-12/2017
Brief description of one of the biggest representative of immunoglobulin superfamily - Down Syndrome Cell Adhesion Molecule (DSCAM). Depicts classification, molecular composition and proposed role of this protein in the proces of synaptic plasticity.
Concise discussion on fear and useful methods of it's detection with rodents use
, Patrycja Pańczyszyn-Trzewik, MSc
Involvement of cyclooxygenase 2 in antidepressant-like effects of metabotropic glutamatergic receptors group I. Grant: SONATA 7 NCN, Katarzyna Stachowicz, PhD
, Piotr Brański, PhD
L'OREAL For Women in Science , Katarzyna Stachowicz, PhD
Monday, 30 November -0001
Scientific Team Award WUM, Katarzyna Stachowicz, PhD
Monday, 30 November -0001
Postdoc Research position Vanderbilt University, Katarzyna Stachowicz, PhD
Monday, 30 November -0001
, Piotr Brański, PhD
Monday, 30 November -0001
- 4 plates test
- active avoidance
- chronic mild stress
- differential centrifugation
- electroconvulsive therapy
- excitotoxic lesions
- forced swim test
- head twitches test
- immobilisation stress
- in situ hybridisation
- LDH test
- locomotor activity
- mammalian cells tissue cultures
- microdialysis in vitro
- model of trangenic knock out
- molecular cloning
- MTT test
- northern blot
- novel obiect recognition
- open field test
- organotypic cultures
- passive avoidance
- point mutations
- primary cultures
- protein fractioning
- quantitative autoradiography
- radiologand binding
- Real Time PCR
- second messenger generation
- social interactions
- spontaneous alternation test
- stereotactic implantation of cannulas
- sucrose preference test
- tail suspension test
- tissue cultures with stable expressing genes encoding selected proteins
- Vogels test
- Western Blot
- allosteric modulation
- behavioural analysis
- Bipolar disorder
- fluorescent microscopy
- G-protein coupled receptors
- GABAB receptors
- glutamatergic receptors
- immune system
- metabotropic glutamate receptors
- molecular biology
- neuropeptide Y
- NMDA receptor
- NPY receptors
- prefrontal cortex
- second messengers
- serotonin receptors
- signaling proteins
- treatment-resistant depression