Department of Neurobiology
The Department of Neurobiology consists of separate research teams and the independent Laboratory of Trace Elements Neurobiology. Research teams conduct complementary studies in areas such as metabotropic receptors for glutamate and the search for new sites of action for psychotropic drugs.
The team consisting of the following members: dr hab. Agnieszka Pałucha-Poniewiera (PhD), Karolina Podkowa (MPharm) and Szymon Rzeźniczek (MSc) takes part in the study of potential antidepressant properties of glutamate metabotropic ligands and their mechanisms of action.
The team consisting of the following members: Piotr Brański (PhD), Grzegorz Burnat (PhD), Paulina Chorobik (PhD), Marcin Marcinak (PhD), Barbara Chruścicka (MSc) deals with an identification and pharmacodynamic characteristics of biologically active chemical compounds. Their studies are focused on metabotropic receptors, in particular mGluR and GABA B.
The methodology and experimental procedures:
- Mammalian cell cultures
- Analysis of protein expression using RT-PCR and Western Blot
- Immunofluorescence (microscopy and FACS)
- Measurement of metabotropic receptors-mediated GTPase activity in isolated cell membranes
- Cyclic ATP measurement (cells, tissue sections) using HTRF
- Kinetic luminometric measurement of intracellular cAMP levels using a molecular sensor (Glo)
- Measurement of the IP1 accumulation (cells, tissue sections) using HTRF
- Measurement of intracellular mobilization of calcium ions (fluorescence)
- Molecular cloning (propagation of bacterial vectors, modification of DNA sequence, transfer into mammalian cells [including tetracycline-inducible gene expression system], selection of cell clones)
- Point mutations
- Experiments on transgenic animals (PCR, electrophoretic separation of products)
- Evaluation of sperm quality parameters (evaluation of: motility, vitality – eosinophilic test, maturity – presence and location of cytoplasmic droplets, integrity of cytoplasmic membrane – hyposmotic test and morphology of spermatozoids)
Dr hab. Joanna Wierońska (PhD), Monika Woźniak (MSc) and Paulina Cieślik (MSc) form a team whose main research direction are antipsychotics and their mechanisms of action. We use animal models of positive, negative and cognitive symptoms as well as techniques to determine the level of specific proteins and mRNA. Our studies oscillate around metabotropic receptors for glutamate and GABA B receptors.
The Laboratory of Neurodegeneration and Neuroprotection (no formal status), whose members are prof. dr hab. Maria Śmiałowska and Dr. Helena Domin, is involved in:
- Research on neurodegeneration and neuroprotection in experimental models of ischemia
- Study of neuroprotective and antidepressant mechanisms of glutamate group III receptor ligands and neuropeptide Y receptor ligands
- Study of anatomical and histochemical interactions between different types of neurons and astrocytes in the cerebral cortex and limbic structures. We pay special attention to GABA and NPY neurons.
- Histology (Nissl, HE, Kluver-Barrera staining)
- Histochemistry and immunohistochemistry (NPY-ir, GAD67-ir, GFAP-ir, CRF-ir, NeuN-ir, TH-ir, TrpH-ir, immunohistochemical staining of selected receptors, and others)
- Stereological quantification in microscopic images
- Intracerebral administration of substances
- Behavioral observations and tests
- Western blot analysis of selected proteins or peptides.
The team closely collaborates with Laboratories of the Department of Neurobiology and other researchers in the Institute, especially in the field of behavior, Western blotting, neural cell cultures, and assessment of neurodegenerative changes in Parkinson's disease models.
The Department also conducts studies regarding the anxiolytic activity of drugs and the mechanisms of action of these compounds.
Neuroprotective effect of the group III mGlu receptor agonist ACPT-I after ischemic stroke in rats with essential hypertension.
Helena Domin, Łukasz Przykaza, Ewa Kozniewska, Paweł M Boguszewski, Maria Śmiałowska
Progress in neuro-psychopharmacology & biological psychiatry, S0278-5846(17)30736-4 10.1016/j.pnpbp.2018.02.006
Comparative effectiveness of abatacept, apremilast, secukinumab and ustekinumab treatment of psoriatic arthritis: a systematic review and network meta-analysis.
P Kawalec, P Holko, P Moćko, A Pilc
Rheumatology international, 10.1007/s00296-017-3919-7
Involvement of extracellular signal-regulated kinase (ERK) in the short and long-lasting antidepressant-like activity of NMDA receptor antagonists (zinc and Ro 25-6981) in the forced swim test in rats.
Bartłomiej Pochwat, Anna Rafało-Ulińska, Helena Domin, Paulina Misztak, Gabriel Nowak, Bernadeta Szewczyk
Neuropharmacology, S0028-3908(17)30373-8 10.1016/j.neuropharm.2017.08.006
The involvement of monoaminergic neurotransmission in the antidepressant-like action of scopolamine in the tail suspension test.
Agnieszka Pałucha-Poniewiera, Karolina Podkowa, Tomasz Lenda, Andrzej Pilc
Progress in neuro-psychopharmacology & biological psychiatry, S0278-5846(17)30220-8 10.1016/j.pnpbp.2017.06.022
Repeated co-treatment with antidepressants and risperidone increases BDNF mRNA and protein levels in rats.
Zofia Rogóż, Katarzyna Kamińska, Patrycja Pańczyszyn-Trzewik, Magdalena Sowa-Kućma
Pharmacological reports : PR, S1734-1140(16)30398-X 10.1016/j.pharep.2017.02.022
Vortioxetine: A review of the pharmacology and clinical profile of the novel antidepressant.
Magdalena Sowa-Kućma, Patrycja Pańczyszyn-Trzewik, Paulina Misztak, Rafał R Jaeschke, Katherine Sendek, Krzysztof Styczeń, Wojciech Datka, Magdalena Koperny
Pharmacological reports : PR, S1734-1140(16)30361-9 10.1016/j.pharep.2017.01.030
Disease activity, quality of life, and indirect costs of ulcerative colitis in Poland.
Paweł Kawalec, Ewa Stawowczyk, Małgorzata Mossakowska, Andrzej Pilc
Przeglad gastroenterologiczny, 10.5114/pg.2017.66324
Safety Profile of Biologic Drugs in the Treatment of Inflammatory Bowel Diseases: A Systematic Review and Network Meta-analysis of Randomized Controlled Trials.
Paweł Moćko, Paweł Kawalec, Andrzej Pilc
Clinical drug investigation, 10.1007/s40261-016-0459-y
Serotonin transporter and receptor ligands with antidepressant activity as neuroprotective and proapoptotic agents.
Zdzislaw Chilmonczyk, Andrzej J Bojarski, Andrzej Pilc, Ingebrigt Sylte
Pharmacological reports : PR, S1734-1140(16)30162-1 10.1016/j.pharep.2017.01.011
Involvement of GABAB Receptor Signaling in Antipsychotic-like Action of the Novel Orthosteric Agonist of the mGlu4 Receptor, LSP4-2022.
Monika Woźniak, Francine Acher, Marcin Marciniak, Magdalena Lasoń-Tyburkiewicz, Piotr Gruca, Mariusz Papp, Andrzej Pilc, Joanna M Wierońska
Neuropeptide Y Y2 and Y5 receptors as promising targets for neuroprotection in primary neurons exposed to oxygen-glucose deprivation and in transient focal cerebral ischemia in rats.
Helena Domin, Łukasz Przykaza, Danuta Jantas, Ewa Kozniewska, Paweł M Boguszewski, Maria Śmiałowska
Neuroscience, S0306-4522(16)30740-0 10.1016/j.neuroscience.2016.12.040
Health-Related Quality of Life Impairment and Indirect Cost of Crohn's Disease: A Self-Report Study in Poland.
Przemysław Holko, Paweł Kawalec, Małgorzata Mossakowska, Andrzej Pilc
PloS one, 10.1371/journal.pone.0168586
Antidepressant-like activity of the neuropeptide Y Y5 receptor antagonist Lu AA33810: behavioral, molecular, and immunohistochemical evidence.
Helena Domin, Bernadeta Szewczyk, Bartłomiej Pochwat, Monika Woźniak, Maria Śmiałowska
The transparency of published health technology assessment-based recommendations on pharmaceutical reimbursement in Poland.
Tomasz Bochenek, Ewa Kocot, Marcin Rodzinka, Brian Godman, Katarzyna Maciejewska, Susan Kamal, Andrzej Pilc
Expert review of pharmacoeconomics & outcomes research, 10.1080/14737167.2017.1262767
SERS characterization of neuropeptide Y and its C-terminal fragments deposited onto colloidal gold nanoparticle surface.
Helena Domin, Natalia Piergies, Dominika Święch, Ewa Pięta, Edyta Proniewicz
Colloids and surfaces. B, Biointerfaces, S0927-7765(16)30704-4 10.1016/j.colsurfb.2016.10.001
, Patrycja Pańczyszyn-Trzewik, MSc
- 4 plates test
- active avoidance
- chronic mild stress
- differential centrifugation
- electroconvulsive therapy
- excitotoxic lesions
- forced swim test
- head twitches test
- immobilisation stress
- in situ hybridisation
- LDH test
- locomotor activity
- mammalian cells tissue cultures
- microdialysis in vitro
- model of trangenic knock out
- molecular cloning
- MTT test
- northern blot
- novel obiect recognition
- open field test
- organotypic cultures
- passive avoidance
- point mutations
- primary cultures
- protein fractioning
- quantitative autoradiography
- radiologand binding
- Real Time PCR
- second messenger generation
- social interactions
- spontaneous alternation test
- stereotactic implantation of cannulas
- sucrose preference test
- tail suspension test
- tissue cultures with stable expressing genes encoding selected proteins
- Vogels test
- Western Blot
- allosteric modulation
- behavioural analysis
- Bipolar disorder
- fluorescent microscopy
- G-protein coupled receptors
- GABAB receptors
- glutamatergic receptors
- immune system
- metabotropic glutamate receptors
- molecular biology
- neuropeptide Y
- NMDA receptor
- NPY receptors
- prefrontal cortex
- second messengers
- serotonin receptors
- signaling proteins
- treatment-resistant depression