Department of Neurochemistry


Research
- Research of new endogenous substances with neuroprotective and antidepressant properties: endogenous tetrahydroisoquinoline amines (TIQ and 1MeTIQ) as potential safe drugs in clinic

- PARKINSON'S DISEASE
Animal models of PD
rotenone
salsolinol
1BnTIQ
inhibitor of VMAT
dopamine metabolism and release (in vivo as a target for neurotoxic and neuroprotective agents)
neurobiological mechanisms underlying depression in Parkinson's disease

- ADDICTION
behavioral and neurochemical correlates of craving and drug dependence (cocaine, morphine)
neuroprotective tetrahydroisoquinolines (TIQ, 1MeTIQ) as neuroprotective, antiaddictive and antidepressant agents
role of dopamine and its extraneuronal metabolite, 3-metoxytyramine (3-MT) in dopamine hyperactivity and drug addiction: behavioral and molecular studies
the neurotoxic effects of endogenous substances which may be responsible for Parkinson’s disease (salsolinol and 1BnTIQ)

Methods
Behavioural:
- locomotor activity
- stereotypy
- catalepsy
- tail flick and hot plate tests
- Porsolt’s test
- instrumental treatments - electroconvulsive shock
Stereotaxic:
- the implantation of chronic cannula into brain structures
- microinjections into the brain
- chemical lesions of the brain
Histological:
- verification of brain lesions and microinjection sites
Biochemical:
- in vitro and ex vivo radioligand binding assay the HPLC with ED analysis of biogenic amines and their metabolites concentrations in the brain structures:
dopamine (DA) and its metabolites (DOPAC, 3-MT, HVA)
noradrenaline (NA) and its metabolites (MOPEG, NM)
serotonin (5-HT) and its metabolite (5-HIAA)
excitatory amino acids (Glu, Asp)
assessment of monoamine metabolic rates
- in vivo microdialysis study and analysis of extracellular biogenic amines and excitatory amino acids in the brain structures of freely moving rat (HPLC Coulochem System with UV detector)
- assay of monoamine tissue uptake
- assay of MAO activity

The Department cooperates with:
Department of Psychiatry and Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA

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