Laboratory of Immunoendocrinology
[Site under construction]
The reduced level of growth factors in an animal model of depression is accompanied by regulated necrosis in the frontal cortex but not in the hippocampus.
Mateusz Kucharczyk, Anna Kurek, Bartosz Pomierny, Jan Detka, Mariusz Papp, Katarzyna Tota, Bogusława Budziszewska
Psychoneuroendocrinology, S0306-4530(17)31488-9 10.1016/j.psyneuen.2018.05.008
Regulators of glucocorticoid receptor function in an animal model of depression and obesity.
Anna Kurek, Katarzyna Głombik, Jan Detka, Agnieszka Basta-Kaim, Marta Kubera, Władysław Lasoń, Bogusława Budziszewska
Journal of neuroendocrinology, 10.1111/jne.12591
Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures.
Anna Kurek, Mateusz Kucharczyk, Jan Detka, Joanna Ślusarczyk, Ewa Trojan, Katarzyna Głombik, Bartosz Bojarski, Agnieszka Ludwikowska, Władysław Lasoń, Bogusława Budziszewska
Neurotoxicity research, 10.1007/s12640-016-9630-8
Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism.
Mateusz Kucharczyk, Anna Kurek, Jan Detka, Joanna Slusarczyk, Mariusz Papp, Katarzyna Tota, Agnieszka Basta-Kaim, Marta Kubera, Wladyslaw Lason, Bogusława Budziszewska
Psychoneuroendocrinology, 10.1016/j.psyneuen.2015.12.019 S0306-4530(15)30045-7
Anti-inflammatory properties of tianeptine on lipopolysaccharide-induced changes in microglial cells involve Toll-like receptor-related pathways.
Joanna Slusarczyk, Ewa Trojan, Katarzyna Glombik, Anna Piotrowska, Boguslawa Budziszewska, Marta Kubera, Katarzyna Popiolek-Barczyk, Wladyslaw Lason, Joanna Mika, Agnieszka Basta-Kaim
Journal of neurochemistry, 10.1111/jnc.13452
The Beneficial Impact of Antidepressant Drugs on Prenatal Stress-Evoked Malfunction of the Insulin-Like Growth Factor-1 (IGF-1) Protein Family in the Olfactory Bulbs of Adult Rats.
Ewa Trojan, Katarzyna Głombik, Joanna Ślusarczyk, Bogusława Budziszewska, Marta Kubera, Adam Roman, Władysław Lasoń, Agnieszka Basta-Kaim
Inhibitory effect of antidepressant drugs on contact hypersensitivity reaction is connected with their suppressive effect on NKT and CD8(+) T cells but not on TCR delta T cells.
Katarzyna Curzytek, Marta Kubera, Monika Majewska-Szczepanik, Marian Szczepanik, Włodzimierz Ptak, Weronika Duda, Monika Leśkiewicz, Agnieszka Basta-Kaim, Bogusława Budziszewska, Magdalena Regulska, Barbara Korzeniak, Katarzyna Głombik, Michael Maes, Władysław Lasoń
International immunopharmacology, 10.1016/j.intimp.2015.08.001 S1567-5769(15)30059-X
Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats.
Katarzyna Głombik, Aneta Stachowicz, Joanna Ślusarczyk, Ewa Trojan, Bogusława Budziszewska, Maciej Suski, Marta Kubera, Władysław Lasoń, Krzysztof Wędzony, Rafał Olszanecki, Agnieszka Basta-Kaim
Psychoneuroendocrinology, 10.1016/j.psyneuen.2015.06.015 S0306-4530(15)00230-9
Toward Omics-Based, Systems Biomedicine, and Path and Drug Discovery Methodologies for Depression-Inflammation Research.
Michael Maes, Gabriel Nowak, Javier R Caso, Juan Carlos Leza, Cai Song, Marta Kubera, Hans Klein, Piotr Galecki, Cristiano Noto, Enrico Glaab, Rudi Balling, Michael Berk
Brain glucose metabolism in an animal model of depression.
J Detka, A Kurek, M Kucharczyk, K Głombik, A Basta-Kaim, M Kubera, W Lasoń, B Budziszewska
Neuroscience, 10.1016/j.neuroscience.2015.03.046 S0306-4522(15)00283-3
Prenatal stress is a vulnerability factor for altered morphology and biological activity of microglia cells.
Joanna Ślusarczyk, Ewa Trojan, Katarzyna Głombik, Bogusława Budziszewska, Marta Kubera, Władysław Lasoń, Katarzyna Popiołek-Barczyk, Joanna Mika, Krzysztof Wędzony, Agnieszka Basta-Kaim
Frontiers in cellular neuroscience, 10.3389/fncel.2015.00082
Prenatal administration of lipopolysaccharide induces sex-dependent changes in glutamic acid decarboxylase and parvalbumin in the adult rat brain.
A Basta-Kaim, K Fijał, J Ślusarczyk, E Trojan, K Głombik, B Budziszewska, M Leśkiewicz, M Regulska, M Kubera, W Lasoń, K Wędzony
Neuroscience, 10.1016/j.neuroscience.2014.12.013 S0306-4522(14)01055-0
Elevated brain glucose and glycogen concentrations in an animal model of depression.
Jan Detka, Anna Kurek, Agnieszka Basta-Kaim, Marta Kubera, Władysław Lasoń, Bogusława Budziszewska
The impact of prenatal stress on insulin-like growth factor-1 and pro-inflammatory cytokine expression in the brains of adult male rats: the possible role of suppressors of cytokine signaling proteins.
Ewa Szczesny, Agnieszka Basta-Kaim, Joanna Slusarczyk, Ewa Trojan, Katarzyna Glombik, Magdalena Regulska, Monika Leskiewicz, Boguslawa Budziszewska, Marta Kubera, Wladyslaw Lason
Journal of neuroimmunology, 10.1016/j.jneuroim.2014.08.001 S0165-5728(14)00234-3
Stress-induced alterations in 5-HT1A receptor transcriptional modulators NUDR and Freud-1.
Bernadeta Szewczyk, Katarzyna Kotarska, Mireille Daigle, Paulina Misztak, Magdalena Sowa-Kucma, Anna Rafalo, Katarzyna Curzytek, Marta Kubera, Agnieszka Basta-Kaim, Gabriel Nowak, Paul R Albert
The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP), 10.1017/S146114571400100X
The influence of glucagon-like peptie-1 receptor agaonists on regulation of coticotropin-releasing hormone gene promoter activity UMO-2012/07/N/NZ7/04394, Jan Detka, PhD
The aim of the project is to determine the effect of glucagon-like peptide-1 receptor (GLP-1R) agonists, used to treat type 2 diabetes on the regulation of corticoliberin (CRH) gene expression. Many reports have indicated a distinct role of GLP-1 as a brain modulator of neuroendocrine processes, emphasizing its implication in hypothalamic-pituitary-adrenal (HPA) axis activation. Considering that chronic HPA axis activation is thought to be an important factor in the pathogenesis of depression which can also stimulate type 2 diabetes development in some depressed patients, it is necessary to better understand GLP-1 involvement in hormonal regulation in the organism and its action in the hypothalamus. Numerous papers suggest GLP-1 involvement in the activation of hypothalamic paraventricular neurons and most recent reports indicate its influence on CRH expression in transiently transfected neuronal cell line. However, so far the effect of GLP-1 on CRH gene expression was not studied in terms protein kinase A activation (main pathway of CRH activation in stress) or attenuation of CRH gene activity by glucocorticoids. It is also undetermined if the potential effect of GLP-1 in the hypothalamus depends on insulin, as well as nobody studied the cellular mechanisms, involved in the following processes.
The role of glucocorticoids in the regulation of neurodegenerative process, Anna Kurek, MSc
Glucocorticoids can produce either protective or neurodegenerative effects, depending on the concentration and duration of action. Experimental data have indicated that long-lastingly increased glucocorticoid level can contribute to neurodegenerative changes observed in depression and after cerebral stroke. However, in opposite to in vivo studies, in a majority of in vitro experiments, neurotoxic action of glucocorticoids was observed only after application of their very high doses. Since in vivo investigations have demonstrated that glucocorticoids influence many types of nervous system cells (neurons, astrocytes, microglia), it appears that the lack of junctions between different cells can be one of causes of their weak cytotoxic effect in in vitro studies, conducted most often in neuronal cultures. Moreover, in a few studies on organotypic cultures carried out so far, the cultures were initiated from tissues collected from control animals while HPA axis activity and strength of glucocorticoid action in adults largely depends on factors acting in perinatal period. The aim of the proposed experiments is to examine a potential, cytotoxic action of corticosterone and its interaction with glutamate in organptypic cultures of the hippocampus obtained from the prenatally stress-exposed animals.
We assume that prenatal stress which changes expression of many factors affecting the function of glucocorticoid and NMDA receptors and glutamate level will alter also the effect of corticosterone on neurodegenerative/neuroprotective processes in the hippocampus. Determination of the effect and mechanism of action of corticosterone added to culture medium on markers of cell damage in organotypic cultures of hippocampi from control and prenatally stressed animals (an animal model of depression) will allow for answering the question whether the changes triggered by prenatally elevated glucocorticoids will sensitize the tissue to damaging factors acting in adulthood.
- forced swim test
- immobilisation stress
- immune cells tissue cultures
- in vitro transfection
- LDH test
- mammalian cells tissue cultures
- MTT test
- novel obiect recognition
- protein fractioning
- radiologand binding
- Real Time PCR
- tissue cultures with stable expressing genes encoding selected proteins
- Western Blot