Department of Experimental Neuroendocrinology
Scope of research:
Evaluation of the effectiveness of chronic antidepressant drug treatments in the hippocampal mitochondria - A proteomic study in an animal model of depression.
Katarzyna Głombik, Aneta Stachowicz, Ewa Trojan, Rafał Olszanecki, Joanna Ślusarczyk, Maciej Suski, Katarzyna Chamera, Bogusława Budziszewska, Władysław Lasoń, Agnieszka Basta-Kaim
Progress in neuro-psychopharmacology & biological psychiatry, S0278-5846(17)30045-3 10.1016/j.pnpbp.2017.05.014
Suppression of pro-inflammatory cytokine expression and lack of anti-depressant-like effect of fluoxetine in lipopolysaccharide-treated old female mice.
Weronika Duda, Marta Kubera, Grzegorz Kreiner, Katarzyna Curzytek, Jan Detka, Katarzyna Głombik, Joanna Ślusarczyk, Agnieszka Basta-Kaim, Bogusława Budziszewska, Władysław Lasoń, Magdalena Regulska, Monika Leśkiewicz, Adam Roman, Agnieszka Zelek-Molik, Irena Nalepa
International immunopharmacology, S1567-5769(17)30155-8 10.1016/j.intimp.2017.04.021
Stimulatory effect of desipramine on lung metastases of adenocarcinoma MADB 106 in stress highly-sensitive and stress non-reactive rats.
Beata Grygier, Marta Kubera, Danuta Wrona, Adam Roman, Agnieszka Basta-Kaim, Piotr Gruca, Mariusz Papp, Zofia Rogoz, Monika Leskiewicz, Boguslawa Budziszewska, Magdalena Regulska, Barbara Korzeniak, Katarzyna Curzytek, Katarzyna Glombik, Joanna Slusarczyk, Michael Maes, Wladyslaw Lason
Progress in neuro-psychopharmacology & biological psychiatry, S0278-5846(17)30063-5 10.1016/j.pnpbp.2017.04.024
Apoptosis Induced by the UV Filter Benzophenone-3 in Mouse Neuronal Cells Is Mediated via Attenuation of Erα/Pparγ and Stimulation of Erβ/Gpr30 Signaling.
A Wnuk, J Rzemieniec, W Lasoń, W Krzeptowski, M Kajta
Molecular neurobiology, 10.1007/s12035-017-0480-z
The ATM kinase inhibitor KU-55933 provides neuroprotection against hydrogen peroxide-induced cell damage via a γH2AX/p-p53/caspase-3-independent mechanism: Inhibition of calpain and cathepsin D.
Jakub Chwastek, Danuta Jantas, Władysław Lasoń
The international journal of biochemistry & cell biology, S1357-2725(17)30067-5 10.1016/j.biocel.2017.03.015
Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling.
Malgorzata Kajta, Agnieszka Wnuk, Joanna Rzemieniec, Ewa Litwa, Wladyslaw Lason, Agnieszka Zelek-Molik, Irena Nalepa, Zofia Rogóż, Adam Grochowalski, Anna K Wojtowicz
The Journal of steroid biochemistry and molecular biology, S0960-0760(17)30066-3 10.1016/j.jsbmb.2017.03.001
Proteomic Analysis of Mitochondria-Enriched Fraction Isolated from the Frontal Cortex and Hippocampus of Apolipoprotein E Knockout Mice Treated with Alda-1, an Activator of Mitochondrial Aldehyde Dehydrogenase (ALDH2).
Aneta Stachowicz, Rafał Olszanecki, Maciej Suski, Katarzyna Głombik, Agnieszka Basta-Kaim, Dariusz Adamek, Ryszard Korbut
International journal of molecular sciences, E435 10.3390/ijms18020435
Regulation of insulin receptor phosphorylation in the brains of prenatally stressed rats: New insight into the benefits of antidepressant drug treatment.
Katarzyna Głombik, Joanna Ślusarczyk, Ewa Trojan, Katarzyna Chamera, Bogusława Budziszewska, Władysław Lasoń, Agnieszka Basta-Kaim
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, S0924-977X(16)32013-2 10.1016/j.euroneuro.2016.12.005
The neuroprotective effects of orthosteric agonists of group II and III mGluRs in primary neuronal cell cultures are dependent on developmental stage.
D Jantas, A Gręda, S Gołda, M Korostyński, W Lasoń
Neuropharmacology, S0028-3908(16)30378-1 10.1016/j.neuropharm.2016.09.003
Dibutyl Phthalate (DBP)-Induced Apoptosis and Neurotoxicity are Mediated via the Aryl Hydrocarbon Receptor (AhR) but not by Estrogen Receptor Alpha (ERα), Estrogen Receptor Beta (ERβ), or Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) in Mouse Cortical Neurons.
Anna K Wójtowicz, Konrad A Szychowski, Agnieszka Wnuk, Małgorzata Kajta
Neurotoxicity research, 10.1007/s12640-016-9665-x
Encapsulation of curcumin in polyelectrolyte nanocapsules and their neuroprotective activity.
Krzysztof Szczepanowicz, Danuta Jantas, Marek Piotrowski, Jakub Staroń, Monika Leśkiewicz, Magdalena Regulska, Władysław Lasoń, Piotr Warszyński
Triclosan activates aryl hydrocarbon receptor (AhR)-dependent apoptosis and affects Cyp1a1 and Cyp1b1 expression in mouse neocortical neurons.
Konrad A Szychowski, Agnieszka Wnuk, Małgorzata Kajta, Anna K Wójtowicz
Environmental research, S0013-9351(16)30302-4 10.1016/j.envres.2016.07.019
Fractalkine Attenuates Microglial Cell Activation Induced by Prenatal Stress.
Joanna Ślusarczyk, Ewa Trojan, Katarzyna Głombik, Katarzyna Chamera, Adam Roman, Bogusława Budziszewska, Agnieszka Basta-Kaim
Neural plasticity, 10.1155/2016/7258201
Beneficial impact of intracerebroventricular fractalkine administration on behavioral and biochemical changes induced by prenatal stress in adult rats: Possible role of NLRP3 inflammasome pathway.
Joanna Ślusarczyk, Ewa Trojan, Karolina Wydra, Katarzyna Głombik, Katarzyna Chamera, Mateusz Kucharczyk, Bogusława Budziszewska, Marta Kubera, Władysław Lasoń, Małgorzata Filip, Agnieszka Basta-Kaim
Biochemical pharmacology, 10.1016/j.bcp.2016.05.008 S0006-2952(16)30081-8
Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures.
Anna Kurek, Mateusz Kucharczyk, Jan Detka, Joanna Ślusarczyk, Ewa Trojan, Katarzyna Głombik, Bartosz Bojarski, Agnieszka Ludwikowska, Władysław Lasoń, Bogusława Budziszewska
Neurotoxicity research, 10.1007/s12640-016-9630-8
Jantas D. Thyreoliberin (TRH) – the regulatory neuropeptide of CNS homeostasis. Advances in Cell Biology 2010, 2(1):138-152.
Molecular mechanism of action of antidepressant drugs in the in vitro model of contact allergy in the human cell line HaCat and mouse dendritic precursor JAWSII cells, MSc Katarzyna Curzytek
The aim of the project is to determine the mechanism of action of antidepressant drugs with different profiles of action engaged in the inhibition of contact hypersensitivity response in two cell lines: the human keratinocyte HaCaT and the mouse dendritic precursor JAWSII. Antidepressants, such as fluoxetine and desipramine proved effective in suppressing contact hypersensitivity (classical example of cell-mediated immune response) in a murine model of contact hypersensitivity. The used antidepressants not only strongly inhibit contact sensitization, but also have shown immunomodulatory effects, but their molecular mechanism of action in inhibition of contact allergy, remains unknown. It is postulated that the use of cellular model for the study of the efficacy of antidepressant drugs in reducing inflammatory response, will contribute to broadening of our knowledge of the intracellular mechanisms of action of these drugs.
, MSc Agnieszka Wnuk
Neurotoxic effects of benzophenone-3 : the role of estrogen receptors and retinoid X receptor alpha.
The impact of maternal diabetes on inflammasome NLRP3 activation in the offspring brain, PhD Katarzyna Głombik
The role of glucocorticoids in the regulation of neurodegenerative process, Anna Kurek
Glucocorticoids can produce either protective or neurodegenerative effects, depending on the concentration and duration of action. Experimental data have indicated that long-lastingly increased glucocorticoid level can contribute to neurodegenerative changes observed in depression and after cerebral stroke. However, in opposite to in vivo studies, in a majority of in vitro experiments, neurotoxic action of glucocorticoids was observed only after application of their very high doses. Since in vivo investigations have demonstrated that glucocorticoids influence many types of nervous system cells (neurons, astrocytes, microglia), it appears that the lack of junctions between different cells can be one of causes of their weak cytotoxic effect in in vitro studies, conducted most often in neuronal cultures. Moreover, in a few studies on organotypic cultures carried out so far, the cultures were initiated from tissues collected from control animals while HPA axis activity and strength of glucocorticoid action in adults largely depends on factors acting in perinatal period. The aim of the proposed experiments is to examine a potential, cytotoxic action of corticosterone and its interaction with glutamate in organptypic cultures of the hippocampus obtained from the prenatally stress-exposed animals.
We assume that prenatal stress which changes expression of many factors affecting the function of glucocorticoid and NMDA receptors and glutamate level will alter also the effect of corticosterone on neurodegenerative/neuroprotective processes in the hippocampus. Determination of the effect and mechanism of action of corticosterone added to culture medium on markers of cell damage in organotypic cultures of hippocampi from control and prenatally stressed animals (an animal model of depression) will allow for answering the question whether the changes triggered by prenatally elevated glucocorticoids will sensitize the tissue to damaging factors acting in adulthood.
, PhD Katarzyna Głombik
Saturday, 21 May 2016
- colorometric evaluation of macrophage activity
- eleveted zero test
- eozynophilic test
- flow cytometry
- forced swim test
- Griess test
- hypoosmotic test
- immobilisation stress
- immune cells tissue cultures
- in vitro transfection
- LDH test
- liquid chromatography
- locomotor activity
- mammalian cells tissue cultures
- MTT test
- novel obiect recognition
- open field test
- organotypic cultures
- prepulse inhibition
- primary cultures
- protein fractioning
- protein microarrays
- radiologand binding
- Real Time PCR
- sucrose preference test
- tail suspension test
- tissue cultures with stable expressing genes encoding selected proteins
- Western Blot
- Alzheimer disease
- behavioural analysis
- Bipolar disorder
- estrogen receptors
- fluorescent microscopy
- G-protein coupled receptors
- gene expression
- glutamatergic receptors
- immobilization stress
- immune system
- metabotropic glutamate receptors
- mitochondrial proteins
- molecular biology
- neuroendocrine regulation
- neurotrophic factors
- NMDA receptor
- Parkinson's disease
- signaling proteins
- xenobiotic receptors