Department of Experimental Neuroendocrinology
Scope of research:
Regulators of glucocorticoid receptor function in an animal model of depression and obesity.
Anna Kurek, Katarzyna Głombik, Jan Detka, Agnieszka Basta-Kaim, Marta Kubera, Władysław Lasoń, Bogusława Budziszewska
Journal of neuroendocrinology, 10.1111/jne.12591
Mitochondrial proteomics investigation of frontal cortex in an animal model of depression: Focus on chronic antidepressant drugs treatment.
Katarzyna Głombik, Aneta Stachowicz, Ewa Trojan, Joanna Ślusarczyk, Maciej Suski, Katarzyna Chamera, Katarzyna Kotarska, Rafał Olszanecki, Agnieszka Basta-Kaim
Pharmacological reports : PR, S1734-1140(17)30491-7 10.1016/j.pharep.2017.11.016
Steroid and Xenobiotic Receptor Signalling in Apoptosis and Autophagy of the Nervous System.
Agnieszka Wnuk, Małgorzata Kajta
International journal of molecular sciences, E2394 10.3390/ijms18112394
Novel ureidopropanamide based N-formyl peptide receptor 2 (FPR2) agonists with potential application for central nervous system disorders characterized by neuroinflammation.
Madia Letizia Stama, Joanna Ślusarczyk, Enza Lacivita, Liliya N Kirpotina, Igor A Schepetkin, Katarzyna Chamera, Chiara Riganti, Roberto Perrone, Mark T Quinn, Agnieszka Basta-Kaim, Marcello Leopoldo
European journal of medicinal chemistry, S0223-5234(17)30737-7 10.1016/j.ejmech.2017.09.023
The Modulatory Properties of Chronic Antidepressant Drugs Treatment on the Brain Chemokine - Chemokine Receptor Network: A Molecular Study in an Animal Model of Depression.
Ewa Trojan, Joanna Ślusarczyk, Katarzyna Chamera, Katarzyna Kotarska, Katarzyna Głombik, Marta Kubera, Agnieszka Basta-Kaim
Frontiers in pharmacology, 10.3389/fphar.2017.00779
Bazedoxifene and raloxifene protect neocortical neurons undergoing hypoxia via targeting ERα and PPAR-γ.
J Rzemieniec, E Litwa, A Wnuk, W Lason, M Kajta
Molecular and cellular endocrinology, S0303-7207(17)30450-1 10.1016/j.mce.2017.08.014
Prenatal stress affects viability, activation, and chemokine signaling in astroglial cultures.
Joanna E Sowa, Joanna Ślusarczyk, Ewa Trojan, Katarzyna Chamera, Monika Leśkiewicz, Magdalena Regulska, Katarzyna Kotarska, Agnieszka Basta-Kaim
Journal of neuroimmunology, S0165-5728(17)30192-3 10.1016/j.jneuroim.2017.08.006
Benzophenone-3 Impairs Autophagy, Alters Epigenetic Status, and Disrupts Retinoid X Receptor Signaling in Apoptotic Neuronal Cells.
Agnieszka Wnuk, Joanna Rzemieniec, Władysław Lasoń, Wojciech Krzeptowski, Małgorzata Kajta
Molecular neurobiology, 10.1007/s12035-017-0704-2
The effects of pessimism on cell-mediated immunity in rats.
Katarzyna Curzytek, Marta Kubera, Ewa Trojan, Kinga Wójcik, Agnieszka Basta-Kaim, Jan Detka, Michael Maes, Rafal Rygula
Progress in neuro-psychopharmacology & biological psychiatry, S0278-5846(17)30059-3 10.1016/j.pnpbp.2017.04.034
Evaluation of the effectiveness of chronic antidepressant drug treatments in the hippocampal mitochondria - A proteomic study in an animal model of depression.
Katarzyna Głombik, Aneta Stachowicz, Ewa Trojan, Rafał Olszanecki, Joanna Ślusarczyk, Maciej Suski, Katarzyna Chamera, Bogusława Budziszewska, Władysław Lasoń, Agnieszka Basta-Kaim
Progress in neuro-psychopharmacology & biological psychiatry, S0278-5846(17)30045-3 10.1016/j.pnpbp.2017.05.014
Suppression of pro-inflammatory cytokine expression and lack of anti-depressant-like effect of fluoxetine in lipopolysaccharide-treated old female mice.
Weronika Duda, Marta Kubera, Grzegorz Kreiner, Katarzyna Curzytek, Jan Detka, Katarzyna Głombik, Joanna Ślusarczyk, Agnieszka Basta-Kaim, Bogusława Budziszewska, Władysław Lasoń, Magdalena Regulska, Monika Leśkiewicz, Adam Roman, Agnieszka Zelek-Molik, Irena Nalepa
International immunopharmacology, S1567-5769(17)30155-8 10.1016/j.intimp.2017.04.021
Stimulatory effect of desipramine on lung metastases of adenocarcinoma MADB 106 in stress highly-sensitive and stress non-reactive rats.
Beata Grygier, Marta Kubera, Danuta Wrona, Adam Roman, Agnieszka Basta-Kaim, Piotr Gruca, Mariusz Papp, Zofia Rogoz, Monika Leskiewicz, Boguslawa Budziszewska, Magdalena Regulska, Barbara Korzeniak, Katarzyna Curzytek, Katarzyna Glombik, Joanna Slusarczyk, Michael Maes, Wladyslaw Lason
Progress in neuro-psychopharmacology & biological psychiatry, S0278-5846(17)30063-5 10.1016/j.pnpbp.2017.04.024
Apoptosis Induced by the UV Filter Benzophenone-3 in Mouse Neuronal Cells Is Mediated via Attenuation of Erα/Pparγ and Stimulation of Erβ/Gpr30 Signaling.
A Wnuk, J Rzemieniec, W Lasoń, W Krzeptowski, M Kajta
Molecular neurobiology, 10.1007/s12035-017-0480-z
The ATM kinase inhibitor KU-55933 provides neuroprotection against hydrogen peroxide-induced cell damage via a γH2AX/p-p53/caspase-3-independent mechanism: Inhibition of calpain and cathepsin D.
Jakub Chwastek, Danuta Jantas, Władysław Lasoń
The international journal of biochemistry & cell biology, S1357-2725(17)30067-5 10.1016/j.biocel.2017.03.015
Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling.
Malgorzata Kajta, Agnieszka Wnuk, Joanna Rzemieniec, Ewa Litwa, Wladyslaw Lason, Agnieszka Zelek-Molik, Irena Nalepa, Zofia Rogóż, Adam Grochowalski, Anna K Wojtowicz
The Journal of steroid biochemistry and molecular biology, S0960-0760(17)30066-3 10.1016/j.jsbmb.2017.03.001
M. Szczęch, K.Szczepanowicz, D.Jantas, M.Piotrowski, A.Kida, W.Lasoń, P.Warszyńsk. Neuroprotective action of undecylenic acid (UDA) encapsulated into PCL nanocarriers. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2017, 532: 41-47
Jantas D. Thyreoliberin (TRH) – the regulatory neuropeptide of CNS homeostasis. Advances in Cell Biology 2010, 2(1):138-152.
Molecular mechanism of action of antidepressant drugs in the in vitro model of contact allergy in the human cell line HaCat and mouse dendritic precursor JAWSII cells, MSc Katarzyna Curzytek
The aim of the project is to determine the mechanism of action of antidepressant drugs with different profiles of action engaged in the inhibition of contact hypersensitivity response in two cell lines: the human keratinocyte HaCaT and the mouse dendritic precursor JAWSII. Antidepressants, such as fluoxetine and desipramine proved effective in suppressing contact hypersensitivity (classical example of cell-mediated immune response) in a murine model of contact hypersensitivity. The used antidepressants not only strongly inhibit contact sensitization, but also have shown immunomodulatory effects, but their molecular mechanism of action in inhibition of contact allergy, remains unknown. It is postulated that the use of cellular model for the study of the efficacy of antidepressant drugs in reducing inflammatory response, will contribute to broadening of our knowledge of the intracellular mechanisms of action of these drugs.
Neurotoxic effects of benzophenone-3 : the role of estrogen receptors and retinoid X receptor alpha., MSc Agnieszka Wnuk
Benzophenone-3 (BP-3) is one of the most commonly used UV filters, which is demonstrated as a hormonally active substance in vitro studies. However, data on the effects of benzophenone-3 on the nervous system are scarce. Especially little is known about the impact of BP-3 on individual receptors that are strongly associated with brain development. The main idea of this project is to investigate the neurotoxic effect of benzophenone-3 on neuronal cells and to establish molecular basis of its activity, with particular emphasis on estrogen receptors and retinoid X receptor alpha. Basic research hypothesis assumes that benzophenone-3 induces a neurotoxic effect and initiates the apoptotic process in the neuronal cells simultaneously modifying activity of the estrogen receptors (ERα, ERβ, GPR30) and retinoid X receptor alpha (RXRα) as well as makes changes in DNA methylation profile.
The impact of maternal diabetes on inflammasome NLRP3 activation in the offspring brain, PhD Katarzyna Głombik
The influence of glucagon-like peptie-1 receptor agaonists on regulation of coticotropin-releasing hormone gene promoter activity UMO-2012/07/N/NZ7/04394, MSc Jan Detka
The aim of the project is to determine the effect of glucagon-like peptide-1 receptor (GLP-1R) agonists, used to treat type 2 diabetes on the regulation of corticoliberin (CRH) gene expression. Many reports have indicated a distinct role of GLP-1 as a brain modulator of neuroendocrine processes, emphasizing its implication in hypothalamic-pituitary-adrenal (HPA) axis activation. Considering that chronic HPA axis activation is thought to be an important factor in the pathogenesis of depression which can also stimulate type 2 diabetes development in some depressed patients, it is necessary to better understand GLP-1 involvement in hormonal regulation in the organism and its action in the hypothalamus. Numerous papers suggest GLP-1 involvement in the activation of hypothalamic paraventricular neurons and most recent reports indicate its influence on CRH expression in transiently transfected neuronal cell line. However, so far the effect of GLP-1 on CRH gene expression was not studied in terms protein kinase A activation (main pathway of CRH activation in stress) or attenuation of CRH gene activity by glucocorticoids. It is also undetermined if the potential effect of GLP-1 in the hypothalamus depends on insulin, as well as nobody studied the cellular mechanisms, involved in the following processes.
The role of glucocorticoids in the regulation of neurodegenerative process, MSc Anna Kurek
Glucocorticoids can produce either protective or neurodegenerative effects, depending on the concentration and duration of action. Experimental data have indicated that long-lastingly increased glucocorticoid level can contribute to neurodegenerative changes observed in depression and after cerebral stroke. However, in opposite to in vivo studies, in a majority of in vitro experiments, neurotoxic action of glucocorticoids was observed only after application of their very high doses. Since in vivo investigations have demonstrated that glucocorticoids influence many types of nervous system cells (neurons, astrocytes, microglia), it appears that the lack of junctions between different cells can be one of causes of their weak cytotoxic effect in in vitro studies, conducted most often in neuronal cultures. Moreover, in a few studies on organotypic cultures carried out so far, the cultures were initiated from tissues collected from control animals while HPA axis activity and strength of glucocorticoid action in adults largely depends on factors acting in perinatal period. The aim of the proposed experiments is to examine a potential, cytotoxic action of corticosterone and its interaction with glutamate in organptypic cultures of the hippocampus obtained from the prenatally stress-exposed animals.
We assume that prenatal stress which changes expression of many factors affecting the function of glucocorticoid and NMDA receptors and glutamate level will alter also the effect of corticosterone on neurodegenerative/neuroprotective processes in the hippocampus. Determination of the effect and mechanism of action of corticosterone added to culture medium on markers of cell damage in organotypic cultures of hippocampi from control and prenatally stressed animals (an animal model of depression) will allow for answering the question whether the changes triggered by prenatally elevated glucocorticoids will sensitize the tissue to damaging factors acting in adulthood.
, MSc Ewa Trojan
, PhD Katarzyna Głombik
Saturday, 21 May 2016
, PhD Joanna Rzemieniec
Monday, 30 November -0001
, PhD Joanna Rzemieniec
Monday, 30 November -0001
- colorometric evaluation of macrophage activity
- eleveted zero test
- eozynophilic test
- flow cytometry
- forced swim test
- Griess test
- hypoosmotic test
- immobilisation stress
- immune cells tissue cultures
- in vitro transfection
- LDH test
- liquid chromatography
- locomotor activity
- mammalian cells tissue cultures
- MTT test
- novel obiect recognition
- open field test
- organotypic cultures
- prepulse inhibition
- primary cultures
- protein fractioning
- protein microarrays
- radiologand binding
- Real Time PCR
- sucrose preference test
- tail suspension test
- tissue cultures with stable expressing genes encoding selected proteins
- Western Blot
- Alzheimer disease
- behavioural analysis
- Bipolar disorder
- estrogen receptors
- fluorescent microscopy
- G-protein coupled receptors
- gene expression
- glutamatergic receptors
- immobilization stress
- immune system
- metabotropic glutamate receptors
- mitochondrial proteins
- molecular biology
- neuroendocrine regulation
- neurotrophic factors
- NMDA receptor
- Parkinson's disease
- signaling proteins
- stem cells
- xenobiotic receptors