Department of Experimental Neuroendocrinology

TECHNIQUES USED

Behavioural research:

Animal models: models of schizophrenia-like behaviours based on maternal immune activation using the administration of lipopolysaccharide or polyinosinic:polycytidylic acid to pregnant female rats, model of depressive-like behaviours based on the prenatal stress procedure, genetic depression model - Wistar Kyoto rats, model of co-occurrence of hypothyroidism and depression, model of Alzheimer’s disease-related characteristics, employing APPNL-F/NL-F knock-in mice, rat diabetes type I model (streptozotocin)

Behavioural tests: forced swim test (FST), prepulse inhibition test (PPI), water maze test, light-dark box test, social interactions test, exploratory and locomotor activities tests, novel object recognition test (NOR), Y-maze test, open field test (OFT), sucrose preference test

 

Methods of cellular biology:

Biochemical assays: cell viability/toxicity, enzymes activities, immunocytochemistry, live-cell imaging, flow cytometry, gene silencing and overexpression

Cell cultures in vitro and ex-vivo: primary neuronal, neuronal-glia, astrocyte and microglia cell cultures, cell lines (SH-SY5Y, U87-MG, C6, HT-22, HaCat, JAWSII), organotypic hippocampal and cortical cell cultures, immune cells tissue cultures

Cellular models: ischemia (OGD), excitotoxicity, oxidative stress, Parkinson’s disease, apoptosis, neuroiflammation, contact allergy

 

Molecular techniques:

Molecular methods to evaluate the levels of proteins or RNA in basic conditions or after exposure to drugs or other stimulants: qRT-PCR, ELISA, Western blot, Luminex


THE MOST IMPORTANT RECENT DISCOVERIES

1.     It has been shown that disturbances in the levels of neuronal and microglial proteins CD200-CD200R and CX3CL1-CX3CR1,as well as the accompamying changes in microglial reactivity, caused by the maternal immune activation during pregnancy, constitute an important element of the complex basis of the development of schizophrenia-like disorders in adulthood.

2.     In hippocampal organotypic cultures, in the ex vivo model of ischemia, dysfunction in the network of chemokines and their receptors, inflammatory activation and disturbed mechanotransduction mechanism, and consequently changes in the rigidity of organotypic cultures have been demonstrated.


FORMER EMPLOYEES OF THE DEPARTMENT:

Professor Bogusława Budziszewska, PhD

Lucylla Jaworska-Feil, PhD

Katarzyna Kotarska, PhD

Magdalena Szuster-Głuszczak, PhD

Magdalena Procner, PhD

Rugmani Meenambal, PhD

Danuta Jantas,PhD

More publications
  • Application of dental pulp stem cells in neural tissue regeneration


More publications
  • OPUS 22. The role of the immune checkpoints in the pathomechanism of depressive disorders., Professor Marta Kubera, PhD
    Grant NCN OPUS 22, consortium with the Jagiellonian University; 2022.10.10.-2025.10.09
  • OPUS 22. New approach to the treatment of Alzheimer's disease targeting resolution of inflammation and modulation of biomechanical properties of microglia by FPR2 hybrid agonists., Professor Agnieszka Basta - Kaim, PhD
    Grant NCN OPUS 22, 2021/43/B/NZ4/01133
  • GRIEG 1. Theranostic nanocarriers for drug delivery in central nervous system disorders., Professor Władysław Lasoń, PhD
    Grant GRIEG 1, 2019/34/H/ST5/00578; 2021.12.13-2024.04.12
  • CANALETTO 2021. Promoting resolution of inflammation as an innovate therapeutic strategy for central nervous system disorders characterized by neuroinflammation., Professor Agnieszka Basta - Kaim, PhD
    Research project between Poland and Italy CANALETTO 2021 PPN/BIT/2021/1/00009 Maj Institute of Pharmacology Polish Academy of Sciences and Università di Bari
  • , Katarzyna Głombik, PhD

  • OPUS 20. New generation teranostatic nanocarriers for detection, diagnosis and neuroprotective treatment of ischemic stroke brain damage., Professor Władysław Lasoń, PhD
    Grant NCN OPUS 20, 2020/39/B/NZ7/01913; 2021.07.16-2025.07.15
  • PRELUDIUM 18: Evaluation of molecular mechanisms of neuroprotective action of dental pulp stromal cells in an ex vivo organotypic model of ischemia, Natalia Bryniarska-Kubiak, PhD
    Principal investigator: Natalia Bryniarska
    Financed by the National Science Center (NCN)
  • Grant Joint Programme – Neurodegenerative Disease Research (JPND) , Professor Agnieszka Basta - Kaim, PhD
    EpiAD: Effect of early and adult-life stress on the brain epigenome: relevance for the occurrence of Alzheimer’s Disease and Diabetes-related dementia. Professor Agnieszka Basta - Kaim, PhD
  • The project of Alzheimer's Association: Drug Development of Pro-resolving ALX/FPR2 Agonists for Alzheimer’s Disease., Professor Agnieszka Basta - Kaim, PhD
    Professor Agnieszka Basta-Kaim, PhD (Poland), professor Enza Lacivita, PhD (Italy), professor Lars-Ove Branderburg, PhD (Germany)
  • HARMONIA 9. Modulation of inflammatory processes using new agonists of the formyl peptide receptors ALX/FPR2 as a new therapeutic strategy of depression., Professor Agnieszka Basta - Kaim, PhD
    Grant NCN HARMONIA 9 2017/26/M/NZ7/01048; 2018.05.24-2022.05.23
  • , Katarzyna Głombik, PhD

  • OPUS 10. The role of the neuron-microglia CX3CL1-CX3CR1 and CD200-CD200R protein systems in molecular mechanisms of antipsychotic drug actions: in vivo and in vitro study in the neurodevelopmental models of schizophrenia., Professor Agnieszka Basta - Kaim, PhD
    Grant NCN OPUS 10 2015/19/B/NZ7/02394; 2016.07.20-2021.07.19
  • The role of the inflammasome NLRP3 in the mechanisms of antidepressant drugs action - studies in the animal model of depression, Ewa Trojan, PhD
    Head of the project: dr Ewa Trojan, NCN 2015/17/N/NZ7/00924 (2016.03.24-2020.03.23)
  • PRLEUDIUM 7: Molecular mechanism of action of antidepressant drugs in the in vitro model of contact allergy in the human cell line HaCat and mouse dendritic precursor JAWSII cells., Katarzyna Curzytek - Malicka, PhD
    Grant NCN PRELUDIUM 7, 2014/13/N/NZ6/00639; 2015.03.10-2019.03.09
  • The impact of maternal diabetes on inflammasome NLRP3 activation in the offspring brain, Katarzyna Głombik, PhD
    T
more_grants
  • Excellence Award for Poster Presentation at the 35th European College of Neuropsychopharmacology (ECNP) Congress, 2022, Vienna, Austria, Kinga Tylek, PhD
    Saturday, 15 October 2022
  • Team Scientific Award of the Division of Medical Sciences of the Polish Academy of Sciences for a series of publications entitled "The importance of dysfunction of selected neuronal and microglial proteins in the prenatal period in the pathomechanisms of schizophrenia – studies in neurodevelopmental models"., Professor Agnieszka Basta - Kaim, PhD
    Thursday, 25 November 2021
  • 3rd place in the competition for a popular science article for young scientists of the Maj Institute of Pharmacology PAS: "Feel the force around you" - how do physical interactions affect cells?, Natalia Bryniarska-Kubiak, PhD
    Thursday, 30 September 2021
  • The "Scientist of the Future 2021" award in the category: Science for a better life in the future, Natalia Bryniarska-Kubiak, PhD
    Tuesday, 28 September 2021
  • Research internship scholarship awarded through the STER – Internationalization of Doctoral Schools program to complete a research internship in the Department of Pharmacy at the University of Bari (Italy), Kinga Tylek, PhD
    Monday, 6 September 2021
  • Travel Grant ENCODS 2021, Federation of European Neuroscience Societies (FENS) Glasgow, United Kingdom, Kinga Tylek, PhD
    Friday, 4 June 2021
  • Scholarship of the President of the Polish Academy of Sciences, Natalia Bryniarska-Kubiak, PhD
    Monday, 30 November 2020
  • Scholarship for the best PhD students at the Maj Institute of Pharmacology, Polish Academy of Sciences (2020/2021), Natalia Bryniarska-Kubiak, PhD
    Friday, 13 November 2020
  • Laureate of the competition organized by "Pharmacological reports" and director of May Institute of Pharmacology Polish Academy of Sciences for the best draft of review article, Natalia Bryniarska-Kubiak, PhD
    Tuesday, 10 November 2020
  • Scholarship of the Minister of Science and Higher Education for outstanding young scientists, Katarzyna Głombik, PhD
    Monday, 15 June 2020
  • The best work in the Pharmacy and Pharmacology session during the 10th National Conference on Advence in Biomedical Research in Warsaw, Natalia Bryniarska-Kubiak, PhD
    Saturday, 30 November 2019
  • The main prize of Professor Kazimierz Ostrowski for the best work presented during the 10th National Conference on Advences in Biomedical Research in Warsaw, Natalia Bryniarska-Kubiak, PhD
    Saturday, 30 November 2019
  • Neuron of The Audience: Best Poster on 9th Annual Conference Aspects of Neuroscience in Warsaw, Natalia Bryniarska-Kubiak, PhD
    Sunday, 24 November 2019
  • Scholarship for the best PhD students at the Maj Institute of Pharmacology Polish Academy of Sciences (2019/2020), Natalia Bryniarska-Kubiak, PhD
    Thursday, 7 November 2019
  • 3rd place in the competition for a popular science article for young scientists of the Polish Academy of Sciences, Natalia Bryniarska-Kubiak, PhD
    Friday, 27 September 2019
more_awards
Head

Professor Agnieszka Basta - Kaim, PhD

Phone number: +48 12 6623273
E-mail: basta@if-pan.krakow.pl

interests

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