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Copyright © 1999 by Institute of Pharmacology Polish Academy of Sciences |
Pol. J. Pharmacol., 1999, 51, 525-530 ISSN 1230-6002 |
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Haloperidol treatment selectively affects expression of Galpha-i3 subunit mRNA in specific regions of the rat brain.
M. SANAK, G. KREINER, M. KOWALSKA, I. NALEPA, J. VETULANI. Pol. J. Pharmacol., 1999, 51, 525-530. Relative abundance of GTP binding protein mRNA coding for subunits alpha-i1, alpha-i2 and aalpha-i3, and their changes induced with chronic blockade of D2 receptor by haloperidol were compared in the frontal cortex, striatum, hippocampus and cerebellar cortex of the rat using a specific method based on multiplex RT-PCR. Galpha-i subtype 2 mRNA was the most abundant, followed by subtype 1 and subtype 3. The haloperidol treatment produced an area specific increase in Gai3 mRNA in the frontal cortex and a decrease of Gai3 in the striatum. After 8-day withdrawal period, haloperidol-induced changes disappeared in the striatum but became accentuated in the frontal cortex. Changes in the relative expression of Gai subtypes may significantly influence long-lasting effects of chronic D2 receptor blockade. Key words: thioridazine metabolism, rat, cytochrome P-450 inhibition, quinine, metyrapone, alpha-naphthoflavone, sulfaphenazole, erythromycin |
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