Copyright © 2000 by Institute of Pharmacology
Polish Academy of Sciences 		Pol. J. Pharmacol., 2000, 52, 299-305
ISSN 1230-6002
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COMPARISON OF MOTOR REACTIVITY OF THE HUMAN COLON COLD-STORED IN DIFFERENT PRESERVATIVE SOLUTIONS TO CARBACHOL AND NORADRENALINE IN VITRO
 Yuichiro Kamikawa1#, Asako Shibukawa1, Kohsuke Uchida1, Shu-ichi Kojima1, Atsushi Sakuma2, Kei-ichi Kubota2, Yasuo Ohno3, Hideki Okudaira4
 Departments of 1 Pharmacology and 2 Surgery II, Dokkyo University School of Medicine, Mibu, Tochigi 321-0293, Japan,
3 Division of Pharmacology, National Institute of Hygienic Sciences, Setagaya, Tokyo 158-8501, Japan,
4 Japan Pharmaceutical Manufacture Association, Nihonbashihoncho, Tokyo 103-0023, Japan

Comparison of motor reactivity of the human colon cold-stored in different preservative solutions to carbachol and noradrenaline in vitro. Y. KAMIKAWA, A. SHIBUKAWA, K. UCHIDA, S. KOJIMA, A. SAKUMA, K. KUBOTA, Y. OHNO, H. OKUDAIRA. Pol. J. Pharmacol., 2000, 52, 299-305.

We have compared the reactivity to carbachol and noradrenaline of circular smooth muscle isolated from the human colon which was cold-stored at 4°C in three different preservative solutions: Krebs bicarbonate solution (KBS), phosphate buffer solution (PBS) or minimum essential medium (MEM). Concentration-dependent contractions in response to carbachol were reduced in terms of both their sensitivity (pEC50) and reactivity (Emax), depending on the period of cold storage. The reduction was more marked when the tissue was cold-stored in either MEM or KBS than in PBS. Similar reduction of the relaxation response to noradrenaline was also observed after cold storage. It is concluded that the cold storage of surgically resected human colon in PBS for two to three days best preserved smooth muscle functions for pharmacological examinations.

Key words: human colon, contraction, relaxation, cold storage, preservative solution, carbachol, noradrenaline
  # correspondence; e-mail: kamikawa@dokkyomed.ac.jp
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