Non-PubMed papers
Bioactive phenolics from in vitro cultures of Lactuca aculeata Boiss. et Kotschy
Stojakowska A., Malarz J. 2017. Phytochem. Lett., 19, 7-11
Sesquiterpenoids from roots of Lactuca sativa var. angustana cv. "Gruener Stern"
Michalska K., Michalski O., Stojakowska A. 2016. DOI 10.1016/j.phytol.2016.10.023
Hydroxycinnamates from elecampane (Inula helenium L.) callus culture
Stojakowska A., Malarz J., Kiss A.K. 2016. Acta Physiol. Plant., 38, 41, DOI 10.1007/s11738-016-2069-y
Dopamine D1 and D2 Receptors in Chronic Mild Stress: Analysis of Dynamic Receptor Changes in an Animal Model of Depression Using In Situ Hybridization and Autoradiography
Depression is a multifaceted illness that involves altered monoamine
neurotransmission. Many monoamine receptor subtypes (e.g., dopamine D1
and D2) demonstrate altered expression levels in depressed patients and
animal models of depression. Currently, there are an increasing number
of molecular and biochemical studies on the mechanism of stress
resilience. In this chapter, we describe a chronic mild stress (CMS)
procedure along with in situ hybridization and autoradiography protocols
to study changes in brain dopamine receptor expression of rats
subjected to CMS. Chronic mild stress procedure (CMS) is one of few
behavioral animal models of depression, and this model has good
construct, face, and predictive validity. Moreover, approximately 30 %
of rats exposed to stress regimen are stress resilient. There are
numerous biochemical techniques that allow to measure changes in
receptor density and the mRNA expression level. Receptor-specific
radioligand binding measures concentration and visualizes the spatial
distribution of the receptor proteins. In situ hybridization is a
specific probe-based semiquantitative histochemical technique that can
be used to visualize the spatial distribution of RNA sequences in tissue
slices.
Dopamine D1 and D2 Receptors in Chronic Mild Stress: Analysis of Dynamic Receptor Changes in an Animal Model of Depression Using In Situ Hybridization and Autoradiography
Depression is a multifaceted illness that involves altered monoamine
neurotransmission. Many monoamine receptor subtypes (e.g., dopamine D1
and D2) demonstrate altered expression levels in depressed patients and
animal models of depression. Currently, there are an increasing number
of molecular and biochemical studies on the mechanism of stress
resilience. In this chapter, we describe a chronic mild stress (CMS)
procedure along with in situ hybridization and autoradiography protocols
to study changes in brain dopamine receptor expression of rats
subjected to CMS. Chronic mild stress procedure (CMS) is one of few
behavioral animal models of depression, and this model has good
construct, face, and predictive validity. Moreover, approximately 30 %
of rats exposed to stress regimen are stress resilient. There are
numerous biochemical techniques that allow to measure changes in
receptor density and the mRNA expression level. Receptor-specific
radioligand binding measures concentration and visualizes the spatial
distribution of the receptor proteins. In situ hybridization is a
specific probe-based semiquantitative histochemical technique that can
be used to visualize the spatial distribution of RNA sequences in tissue
slices.
Acylated hydroxycinnamic acid glucosides from flowers of Telekia speciosa
Stojakowska A., Malarz J., Żylewski M., Kisiel W. 2015. Phytochemistry Lett., 12, 257-261
Rational design of 5-HT6R ligands using a bioisosteric strategy: synthesis, biological evaluation and molecular modelling.
Jakub Staroń, Dawid Warszycki, Justyna
Kalinowska-Tłuścik, Grzegorz Satała, Andrzej J. Bojarski. RSC
Advances, 2015, 5,
25806-25815, DOI: 10.1039/c5ra00054h
Chemical constituents of Lactuca dregeana
Michalska K., Kisiel W., Stojakowska A. 2015. Biochem. Syst. Ecol., 59, 302-304.
