Non-PubMed papers

https://www.sciencedirect.com/science/article/pii/S0925963517305678



Bioactive phenolics from in vitro cultures of Lactuca aculeata Boiss. et Kotschy Stojakowska A., Malarz J. 2017. Phytochem. Lett., 19, 7-11
Sesquiterpenoids from roots of Lactuca sativa var. angustana cv. "Gruener Stern"

Michalska K., Michalski O., Stojakowska A. 2016. DOI 10.1016/j.phytol.2016.10.023



Hydroxycinnamates from elecampane (Inula helenium L.) callus culture

Stojakowska A., Malarz J., Kiss A.K. 2016. Acta Physiol. Plant., 38, 41, DOI 10.1007/s11738-016-2069-y
Dopamine D1 and D2 Receptors in Chronic Mild Stress: Analysis of Dynamic Receptor Changes in an Animal Model of Depression Using In Situ Hybridization and Autoradiography Depression is a multifaceted illness that involves altered monoamine neurotransmission. Many monoamine receptor subtypes (e.g., dopamine D1 and D2) demonstrate altered expression levels in depressed patients and animal models of depression. Currently, there are an increasing number of molecular and biochemical studies on the mechanism of stress resilience. In this chapter, we describe a chronic mild stress (CMS) procedure along with in situ hybridization and autoradiography protocols to study changes in brain dopamine receptor expression of rats subjected to CMS. Chronic mild stress procedure (CMS) is one of few behavioral animal models of depression, and this model has good construct, face, and predictive validity. Moreover, approximately 30 % of rats exposed to stress regimen are stress resilient. There are numerous biochemical techniques that allow to measure changes in receptor density and the mRNA expression level. Receptor-specific radioligand binding measures concentration and visualizes the spatial distribution of the receptor proteins. In situ hybridization is a  specific probe-based semiquantitative histochemical technique that can be used to visualize the spatial distribution of RNA sequences in tissue slices.
Dopamine D1 and D2 Receptors in Chronic Mild Stress: Analysis of Dynamic Receptor Changes in an Animal Model of Depression Using In Situ Hybridization and Autoradiography Depression is a multifaceted illness that involves altered monoamine neurotransmission. Many monoamine receptor subtypes (e.g., dopamine D1 and D2) demonstrate altered expression levels in depressed patients and animal models of depression. Currently, there are an increasing number of molecular and biochemical studies on the mechanism of stress resilience. In this chapter, we describe a chronic mild stress (CMS) procedure along with in situ hybridization and autoradiography protocols to study changes in brain dopamine receptor expression of rats subjected to CMS. Chronic mild stress procedure (CMS) is one of few behavioral animal models of depression, and this model has good construct, face, and predictive validity. Moreover, approximately 30 % of rats exposed to stress regimen are stress resilient. There are numerous biochemical techniques that allow to measure changes in receptor density and the mRNA expression level. Receptor-specific radioligand binding measures concentration and visualizes the spatial distribution of the receptor proteins. In situ hybridization is a  specific probe-based semiquantitative histochemical technique that can be used to visualize the spatial distribution of RNA sequences in tissue slices.