Jan Detka, PhD
Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures.
Anna Kurek, Mateusz Kucharczyk, Jan Detka, Joanna Ślusarczyk, Ewa Trojan, Katarzyna Głombik, Bartosz Bojarski, Agnieszka Ludwikowska, Władysław Lasoń, Bogusława Budziszewska
Neurotoxicity research, 10.1007/s12640-016-9630-8
Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism.
Mateusz Kucharczyk, Anna Kurek, Jan Detka, Joanna Slusarczyk, Mariusz Papp, Katarzyna Tota, Agnieszka Basta-Kaim, Marta Kubera, Wladyslaw Lason, Bogusława Budziszewska
Psychoneuroendocrinology, 10.1016/j.psyneuen.2015.12.019 S0306-4530(15)30045-7
Brain glucose metabolism in an animal model of depression.
J Detka, A Kurek, M Kucharczyk, K Głombik, A Basta-Kaim, M Kubera, W Lasoń, B Budziszewska
Neuroscience, 10.1016/j.neuroscience.2015.03.046 S0306-4522(15)00283-3
Elevated brain glucose and glycogen concentrations in an animal model of depression.
Jan Detka, Anna Kurek, Agnieszka Basta-Kaim, Marta Kubera, Władysław Lasoń, Bogusława Budziszewska
The effect of active and passive intravenous cocaine administration on the extracellular signal-regulated kinase (ERK) activity in the rat brain.
Joanna Miszkiel, Jan Detka, Joanna Cholewa, Małgorzata Frankowska, Ewa Nowak, Bogusława Budziszewska, Edmund Przegaliński, Małgorzata Filip
Pharmacological reports : PR, 10.1016/j.pharep.2014.02.001 S1734-1140(14)00030-9
The influence of glucagon-like peptie-1 receptor agaonists on regulation of coticotropin-releasing hormone gene promoter activity UMO-2012/07/N/NZ7/04394 - 2013-07-04 - 2017-04-03
The aim of the project is to determine the effect of glucagon-like peptide-1 receptor (GLP-1R) agonists, used to treat type 2 diabetes on the regulation of corticoliberin (CRH) gene expression. Many reports have indicated a distinct role of GLP-1 as a brain modulator of neuroendocrine processes, emphasizing its implication in hypothalamic-pituitary-adrenal (HPA) axis activation. Considering that chronic HPA axis activation is thought to be an important factor in the pathogenesis of depression which can also stimulate type 2 diabetes development in some depressed patients, it is necessary to better understand GLP-1 involvement in hormonal regulation in the organism and its action in the hypothalamus. Numerous papers suggest GLP-1 involvement in the activation of hypothalamic paraventricular neurons and most recent reports indicate its influence on CRH expression in transiently transfected neuronal cell line. However, so far the effect of GLP-1 on CRH gene expression was not studied in terms protein kinase A activation (main pathway of CRH activation in stress) or attenuation of CRH gene activity by glucocorticoids. It is also undetermined if the potential effect of GLP-1 in the hypothalamus depends on insulin, as well as nobody studied the cellular mechanisms, involved in the following processes.
- forced swim test
- immobilisation stress
- immune cells tissue cultures
- in vitro transfection
- mammalian cells tissue cultures
- MTT test
- novel obiect recognition
- protein fractioning
- radiologand binding
- Real Time PCR
- tissue cultures with stable expressing genes encoding selected proteins
- Western Blot