Department of Neuropsychopharmacology
- stereotaxic brain operations in rats and intracerebral administration of compounds
- behavioral methods:- automatic measurement of locomotor activity, action tremor and other forms of rat motor behavior in actometers and tremorometers- automatic measurement of asymmetric behavior in rats following unilateral damage of the dopamine system (rotameters)- observation of rats’ behavior: catalepsy test (a model of akinesia, bradykinesia), measurement of tremulous jaw movements (a model of parkinsonian tremor), cylinder test (motor coordination test), dyskinesias
- running as a regular physical exercise training in automatic runways
- histological methods: histological and immunohistochemical staining of brain sections, light and fluorecence microscopy, stereology, morphology anlysis, Sholl method
- biochemical methods: HPLC, brain microdialysis, autoradiography of receptors and transporters, measurement of energy substrates concentration in tissues and blood plasma
- molecular methods: Western blot, in situ hybridization, PCR, analysis of the mitochondrial respiratory complexes activity, 2-dimetional electrophoresis, DIGE
- computer imaging of brain sections, densitometry
Introduction to the Special Section on
Katarzyna Głombik, Katarzyna Kuter-Nowak
Pharmacological reports : PR, 10.1007/s43440-022-00421-9
The Pathophysiology and Treatment of Essential Tremor: The Role of Adenosine and Dopamine Receptors in Animal Models.
Barbara Kosmowska, Jadwiga Wardas
Biomolecules, 1813 10.3390/biom11121813
The influence of preconditioning with low dose of LPS on paraquat-induced neurotoxicity, microglia activation and expression of α-synuclein and synphilin-1 in the dopaminergic system.
Katarzyna Z Kuter, Maria Śmiałowska, Krystyna Ossowska
Pharmacological reports : PR, 10.1007/s43440-021-00340-1
Barbara Kosmowska, Krystyna Ossowska, Jadwiga Wardas
Behavioural brain research, S0166-4328(21)00473-3 10.1016/j.bbr.2021.113585
Beyond the GFAP-Astrocyte Protein Markers in the Brain.
Agnieszka M Jurga, Martyna Paleczna, Justyna Kadluczka, Katarzyna Z Kuter
Biomolecules, 1361 10.3390/biom11091361
Antiparkinsonian-like effects of CPL500036, a novel selective inhibitor of phosphodiesterase 10A, in the unilateral rat model of Parkinson's disease.
Tomasz Lenda, Krystyna Ossowska, Klemencja Berghauzen-Maciejewska, Mikołaj Matłoka, Jerzy Pieczykolan, Maciej Wieczorek, Jolanta Konieczny
European journal of pharmacology, S0014-2999(21)00614-2 10.1016/j.ejphar.2021.174460
Increased Beta-Hydroxybutyrate Level Is Not Sufficient for the Neuroprotective Effect of Long-Term Ketogenic Diet in an Animal Model of Early Parkinson's Disease. Exploration of Brain and Liver Energy Metabolism Markers.
Katarzyna Z Kuter, Łukasz Olech, Urszula Głowacka, Martyna Paleczna
International journal of molecular sciences, 7556 10.3390/ijms22147556
Overview of General and Discriminating Markers of Differential Microglia Phenotypes.
Agnieszka M Jurga, Martyna Paleczna, Katarzyna Z Kuter
Frontiers in cellular neuroscience, 10.3389/fncel.2020.00198
Potential antipsychotic action of the selective agonist of adenosine A1 receptors, 5'-Cl-5'-deoxy-ENBA, in amphetamine and MK-801 rat models.
Krystyna Ossowska, Barbara Kosmowska, Jadwiga Wardas
Pharmacological reports : PR, 10.1007/s43440-020-00093-3
The role of glia in Parkinson's disease: Emerging concepts and therapeutic applications.
Katarzyna Z Kuter, M Angela Cenci, Anna R Carta
Progress in brain research, S0079-6123(20)30021-2 10.1016/bs.pbr.2020.02.004
Pramipexole Reduces zif-268 mRNA Expression in Brain Structures involved in the Generation of Harmaline-Induced Tremor.
Barbara Kosmowska, Krystyna Ossowska, Jadwiga Wardas
Neurochemical research, 10.1007/s11064-020-03010-5
Inhibition of Excessive Glutamatergic Transmission in the Ventral Thalamic Nuclei by a Selective Adenosine A1 Receptor Agonist, 5'-Chloro-5'-Deoxy-(±)-ENBA Underlies its Tremorolytic Effect in the Harmaline-Induced Model of Essential Tremor.
Barbara Kosmowska, Krystyna Ossowska, Jolanta Konieczny, Tomasz Lenda, Klemencja Berghauzen-Maciejewska, Jadwiga Wardas
Neuroscience, S0306-4522(20)30003-8 10.1016/j.neuroscience.2019.12.045
Zona incerta as a therapeutic target in Parkinson's disease.
Journal of neurology, 10.1007/s00415-019-09486-8
Increased energetic demand supported by mitochondrial electron transfer chain and astrocyte assistance is essential to maintain the compensatory ability of the dopaminergic neurons in an animal model of early Parkinson's disease.
Katarzyna Z Kuter, Łukasz Olech, Norbert A Dencher
Mitochondrion, S1567-7249(18)30095-3 10.1016/j.mito.2018.12.002
Astrocyte support is important for the compensatory potential of the nigrostriatal system neurons during early neurodegeneration.
Katarzyna Kuter, Łukasz Olech, Urszula Głowacka, Martyna Paleczna
Journal of neurochemistry, 10.1111/jnc.14605
“Control of Motor Function by Adenosine A2A Receptors in Parkinson’s and Huntington’s Disease”
chapter in the book:
Pinna A., Wardas J., Domenici M. R., Popoli P., Cossu G. and Morelli M. “Control of Motor Function by Adenosine A2A Receptors in Parkinson’s and Huntington’s Disease” In: Blum D, Lopes LV, eds., "Adenosine Receptors in Neurodegenerative Diseases", San Diego: Academic Press, 2017: 188-214.
Adenosine A2A receptors: Localization and Function.
Simola N. and WARDAS J.: Adenosine A2A receptors: Localization and Function. (chapter 1)
In: “The Adenosinergic System. A non-dopaminergic target in Parkinson’s disease” Eds. Morelli M, Simola N., Wardas J. Springer International Publishing Switzerland, 2015, pp. 1-25; Current Topics in Neurotoxicity, vol. 10 (Series Eds. Kostrzewa R., Archer T.).
Kuter, Cenci, Carta, The role of glia in Parkinson's disease: Emerging concepts and therapeutic applications, Progress in Brain Research Volume 252, 2020, Pages 131-168
Kuter K.Z., Cenci M.A., Carta A.R.
"The role of glia in Parkinson's disease: Emerging concepts and therapeutic applications"
Progress in Brain Research Volume 252, 2020, Pages 131-168part of Recent Advances in Parkinson's Disease, edited by Björklund and CenciElsevier
Astrocytes and microglia in Parkinson’s disease and its animal models. In: The Neuroscience of Parkinson's Disease. Vol 2. Genetics, neurology, behavior, and diet in Parkinson’s disease, 2020, Chapter 6, pp 83-101
Astrocytes and microglia in Parkinson’s disease and its animal models. In: The Neuroscience of Parkinson's Disease. Vol 2. Genetics, neurology, behavior, and diet in Parkinson’s disease, 2020, Chapter 6, pp 83-101, Editors: Colin Martin and Victor Preedy, ISBN: 978-0-12-815950-7, published by Academic Press, Imprint of Elsevier, Published Date: 18th September 2020
Stimulation of adenosine A1 receptors and the apoptosis processes in the harmaline model of essential tremor - preliminary studies, Barbara Kosmowska, PhD
NCN Miniatura 5 grant (2021/05/X/NZ7/00057)
grant NCN OPUS18 2019/35/B/NZ7/02862 Physical exercise as a natural mechanism inducing neuroregeneration. Search for the new protein markers and therapeutic targets for Parkinson’s disease, Katarzyna Kuter - Nowak, PhD
, Katarzyna Kuter - Nowak, PhD
NCN grant, OPUS 14 [2018-08-03 - 2021-08-02]
Title: "How microglia-astrocyte interaction affects neuronal functioning
in the dopaminergic system. Analysis of protective potential of drugs changing the glial phenotype"
Motor thalamic nuclei as a potential therapeutic target for adenosine in parkinsonian and essential tremor; behavioural, neurochemical and molecular studies in rats, Professor Jadwiga Wardas, PhD
1. Research project objectives/Research hypothesis Essential (ET) and parkinsonian (PD) tremors are relatively frequent neurological disorders in humans. Since mechanisms underlying these tremors are only poorly understood, current pharmacological therapies of these disturbances are highly insufficient. Therefore, there is an urgent need to search for these mechanisms and find out drugs which would be effective at the clinic.The ventral motor thalamus seems to be a brain structure crucial to relay and integrate impulses related to both PD tremor and ET. This region receives glutamatergic inputs from the cerebellum and GABAergic projections from the basal ganglia and therefore seems to be important for convergence of the cerebellar and basal ganglia informations. The present project is devoted to examine whether adenosine and its receptors may be a target for future pharmacotherapy of ET and/or PD tremor. Since adenosine receptors (A1 and A2A) are present in neuronal networks of the ventral thalamic nuclei, the project is focused at examination of a modulatory role of adenosine receptor ligands on ET and PD tremor in animal models and a contribution of the cerebello-thalamic vs basal ganglia-thalamic circuits in these phenomena.
, Katarzyna Kuter - Nowak, PhD
“MOBILNOSC PLUS” scholarship sponsored by Polish Ministry of Science and Higher Education.
Title: „Protective role of astrocytes in the process of neurodegeneration of dopaminergic cells and during functional compensation of this process. Analysis of function and architecture of mitochondrial oxphos complexes and supercomplexes in animal models of early Morbus Parkinson.”
Performed during three 6 month visits at Technische Universität Darmstadt, Darmstadt, Germany in group of Prof. Norbert A. Dencher.
NCN grant, OPUS 3, Katarzyna Kuter - Nowak, PhD
OPUS 3 grant nr 2012/05/B/NZ4/02599 financed by National Science Centre (NCN).
Title: „The role of astrocytes and cellular metabolism in functional compensation of degeneration of dopaminergic system in threats brain. Analysis of expression of AMPK and energy sensing proteins in aspect of Parkinson's disease.”
Performed in the Institute of Pharmacology, Polish Academy of Sciences – project leader.
Team Scientific Award of the 5th Medical Sciences PAS for a series of 3 publications "The role of astrocytes in mechanisms compensating for the degeneration of dopaminergic neurons and in the regulation of cellular energy of the black-striatal system in an animal model of early Parkinson's disease"., Katarzyna Kuter - Nowak, PhD
Wednesday, 11 December 2019
, Professor Jadwiga Wardas, PhD
Thursday, 14 December 2017
Award for publication woth high IF in 2018 granted by the Head of the Institute of Pharmacology, Katarzyna Kuter - Nowak, PhD
Monday, 30 November -0001
- adenosine receptors
- animal models
- behavioural analysis
- glutamatergic receptors
- metabotropic glutamate receptors
- mitochondrial proteins
- molecular biology
- neurotrophic factors
- nigro-striatal dopaminergic system
- Parkinson's disease